本文選題：微針 + 超聲導(dǎo)入��； 參考：《國際藥學(xué)研究雜志》2017年05期

【摘要】：目的比較微針、超聲導(dǎo)入和離子導(dǎo)入3種物理方式促藥物吸收技術(shù)對芥子堿硫氰酸鹽(ST)體外透皮吸收的效果,探討不同物理方式促進(jìn)透皮吸收的規(guī)律。方法采用改良Franz擴(kuò)散池和SD大鼠腹部皮膚,分別在微針、超聲導(dǎo)入或離子導(dǎo)入條件下進(jìn)行透皮實(shí)驗(yàn)。以2 mg/ml ST水溶液為供體溶液,定時(shí)從接收池中取樣,用高效液相色譜法(HPLC)測定不同時(shí)間點(diǎn)樣品中藥物含量,計(jì)算累積透過量和穩(wěn)態(tài)透皮吸收速度。取各促透皮技術(shù)處理12 h后的皮膚制作病理切片,觀察不同促透技術(shù)對皮膚結(jié)構(gòu)的影響,探討其促吸收機(jī)制。結(jié)果單純透皮條件下,ST累積透過量較少。不同促透技術(shù)對ST有較明顯促透效果,排序如下:微針超聲導(dǎo)入離子導(dǎo)入。微針在皮膚表面形成實(shí)質(zhì)性孔道,促進(jìn)ST透皮吸收的效果最明顯。超聲導(dǎo)入通過擾亂皮膚角質(zhì)層的緊密結(jié)構(gòu)促透。離子導(dǎo)入對皮膚結(jié)構(gòu)影響較小。結(jié)論本研究為不同透皮給藥促透技術(shù)的選擇提供了依據(jù)。
[Abstract]:Objective to compare the effects of microneedle, ultrasonic and iontophoresis on the transdermal absorption of sinapine thiocyanate STs in vitro, and to explore the rules of promoting transdermal absorption by different physical methods. Methods Transdermal experiments were carried out in the modified Franz diffusion pool and the abdominal skin of SD rats under the conditions of microneedle, ultrasound or iontophoresis, respectively. Taking 2 mg/ml St aqueous solution as donor solution, the drug content in different time points was determined by high performance liquid chromatography (HPLC), and the cumulative transmittance and steady transdermal absorption rate were calculated. The pathological sections of the skin were made after 12 h treatment with various transdermal techniques. The effects of different penetration promoting techniques on the skin structure were observed and the mechanism of promoting skin absorption was discussed. Results the cumulative transmittance of St was less under the condition of pure transdermal penetration. The effects of different penetration enhancement techniques on St were as follows: microneedle ultrasonic introduction of ions. The microneedle formed a solid pore on the skin surface, and the effect of St transdermal absorption was the most obvious. Ultrasound leads to penetration by disturbing the tight structure of the cuticle. Iontophoresis has little effect on skin structure. Conclusion this study provides a basis for the selection of different transdermal drug permeation techniques.
【作者單位】： 山東中醫(yī)藥大學(xué)藥學(xué)院;軍事醫(yī)學(xué)科學(xué)院放射與輻射醫(yī)學(xué)研究所;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(81441095)
【分類號】：R943
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本文編號：1901525