本文選題：急進(jìn)高原 + 醋甲唑胺�。� 參考：《中國(guó)藥學(xué)雜志》2015年19期

【摘要】：目的探究急進(jìn)4 300 m高原對(duì)Wistar大鼠機(jī)體血?dú)馍淖�、病理變化、血清腫瘤壞死因子-α和白細(xì)胞介素-1β影響及醋甲唑胺干預(yù)對(duì)大鼠高原損傷的保護(hù)作用。方法將28只成年健康Wistar大鼠按體重隨機(jī)分為平原(海拔55米)對(duì)照組、高原(海拔4 300 m)模型組、醋甲唑胺組、乙酰唑胺組,分別灌胃給予0.9%氯化鈉注射液、醋甲唑胺(每日2次,劑量為2.23 mg·kg-1)和乙酰唑胺(每日2次,劑量為22.33 mg·kg-1),對(duì)大鼠進(jìn)行生化、血?dú)夥治�、病理比較,并檢測(cè)所得血清樣品腫瘤壞死因子-α和白細(xì)胞介素-1β含量。結(jié)果血?dú)�、生化和病理結(jié)果顯示,高原組大鼠出現(xiàn)脫水癥狀,與平原對(duì)照組比較,高原模型組各指標(biāo)變化顯著(P0.01),與高原模型組比較,醋甲唑胺組和乙酰唑胺組門冬氨酸轉(zhuǎn)氨酶(AST)、丙氨酸氨基轉(zhuǎn)移酶(ALT)、酸堿度(p H)、碳酸氫根離子濃度、緩沖堿(BB)、剩余堿(BE)等指標(biāo)顯著下降(P0.01),總蛋白(TP)、尿素(UREA)、二氧化碳分壓(Pa CO2)、鈉離子濃度、氯離子濃度等指標(biāo)顯著上升(P0.01),表明兩組均出現(xiàn)代謝性酸中毒合并呼吸性堿中毒,且肝肺組織出現(xiàn)病理性損傷,其中,與乙酰唑胺組比較,醋甲唑胺組損傷較小。與平原對(duì)照組相比,高原組血清腫瘤壞死因子-α顯著性升高,白細(xì)胞介素-1β顯著性降低,其中,乙酰唑胺組和醋甲唑胺組血清腫瘤壞死因子-α、白細(xì)胞介素-1β含量均顯著高于模型組(P0.01)。結(jié)論醋甲唑胺能改善急進(jìn)高原大鼠的生理生化狀況,減輕炎癥損傷,具有較好的缺氧保護(hù)作用。
[Abstract]:Objective to investigate the changes of blood gas biochemistry, pathological changes, serum tumor necrosis factor- 偽 (TNF- 偽) and interleukin-1 尾 (IL-1- 尾) in Wistar rats at a rapid altitude of 4 300m and the protective effect of alacetazolamine on high altitude injury in rats. Methods 28 healthy adult Wistar rats were randomly divided into plain (55 meters above sea level) control group, high altitude (4 300 m) model group, acetozolamide group and acetazolamide group by intragastric administration of 0.9% sodium chloride injection. The contents of tumor necrosis factor- 偽 and interleukin-1 尾 in serum samples of rats were determined by biochemical analysis, blood gas analysis, pathological comparison, and acetazolamide (2.23 mg 路kg ~ (-1) and acetazolamide (22.33 mg 路kg ~ (-1), 2 times daily. Results the blood gas, biochemical and pathological results showed that the rats in the plateau group had dehydration symptoms. Compared with the plain control group, the indexes of the high altitude model group changed significantly (P 0.01), and those of the plateau model group were higher than those of the plateau model group. Acetazolamide and acetazolamide groups aspartate aminotransferase, alanine aminotransferase, acidazolamide group and acetazolamide group, aspartate transaminase, alanine aminotransferase, alkalinity, hydrogen carbonate ion concentration, The indexes such as buffered base BBN, residual alkaloid (BEB), total protein TPN, urea urEAA, carbon dioxide partial pressure, sodium ion concentration and chloride ion concentration increased significantly (P 0.01), which indicated that metabolic acidosis combined with respiratory alkalosis occurred in both groups. Pathological injury was found in liver and lung tissue, and the injury was less in acetazolamide group than in acetazolamide group. Compared with plain control group, serum tumor necrosis factor- 偽 (TNF- 偽) and interleukin-1 尾 (IL-1- 尾) in high altitude group were significantly increased. The serum levels of tumor necrosis factor- 偽 and interleukin-1 尾 in acetazolamide group and methazolamide group were significantly higher than those in model group (P 0.01). Conclusion Acetazolamide can improve the physiological and biochemical status and reduce the inflammatory injury of rats. It has a better protective effect on hypoxia.
【作者單位】： 蘭州軍區(qū)蘭州總醫(yī)院全軍高原環(huán)境損傷防治重點(diǎn)實(shí)驗(yàn)室;武漢市普仁醫(yī)院;
【基金】：國(guó)家科技部重大資助項(xiàng)目(2008ZXJ09014-010) 全軍醫(yī)藥科研“十二五”重點(diǎn)資助項(xiàng)目(BWS12J012) 全軍醫(yī)藥科研“十二五”面上項(xiàng)目(CWS11C231)
【分類號(hào)】：R965

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 樊軍;秦俊;劉春霞;唐曉蕎;田輝;劉瑤;孫凡中;樊柏林;;建立Wistar大鼠血液及生化指標(biāo)正常參考值范圍的探討[J];公共衛(wèi)生與預(yù)防醫(yī)學(xué);2010年06期

2 Hivana Patricia Melo Barbosa;Luisa Caricio Martins;Sidney Emanuel Batista dos Santos;Samia Demachki;M，

本文編號(hào)：1894366