本文選題：小檗堿 + 克霉唑��； 參考：《中國抗生素雜志》2015年05期

【摘要】：目的探討小檗堿(berberine,BBR)與克霉唑(clotrimazole,CLZ)聯(lián)合用藥時,對白念珠菌(Candida albicans,CA)標(biāo)準(zhǔn)菌株ATCC 10231的體外抑菌效果。方法采用瓊脂擴(kuò)散法(Kirby-Bauer disk diffusion method,KB法)測定BBR、CLZ單用時對CA-10231的最低抑菌濃度(minimum inhibitory concentrations,MIC);把不同濃度BBR與CLZ按棋盤法設(shè)計配制加藥溶液,采用KB法測定不同濃度BBR與CLZ聯(lián)用時對CA-10231的MIC;計算部份抑菌濃度指數(shù)(fractional inhibitory concentration index,FICI),判斷聯(lián)合抑菌效果,FICI≤0.5為協(xié)同作用,0.5FICI≤4為加成作用,FICI4為拮抗作用。結(jié)果 BBR和CLZ單用時對CA-10231的平均MIC分別為32000和32μg/m L;不同濃度BBR與CLZ聯(lián)用時對CA-10231的平均MIC分別為2000μg/m L(P0.01)、8μg/m L(P0.01),平均FICI為0.3125。結(jié)論 BBR、CLZ單用時都能抑制CA-10231生長;BBR與CLZ聯(lián)用時能夠以低劑量藥物協(xié)同抑制CA-10231生長。
[Abstract]:Objective to investigate the bacteriostatic effect of berberine (BBR) combined with clotrimazoleine (clotrimazoleine) on Candida albicans-CA-standard strain ATCC 10231 in vitro. Methods the minimum inhibitory concentration of CA-10231 was determined by Kirby-Bauer disk diffusion method KB method, and the concentration of different concentrations of BBR and CLZ was designed and prepared according to the chessboard method. By using KB method to determine the effect of different concentrations of BBR and CLZ on CA-10231, to calculate the fractional inhibitory concentration index of FICI, and to judge the synergistic effect of FICI 鈮，

本文編號：1894111