本文選題：正電子發(fā)射斷層顯像 + 顯像劑合成��； 參考：《浙江大學》2014年碩士論文

【摘要】：顯像劑是正電子發(fā)射斷層顯像(PET)與核醫(yī)學的關(guān)鍵。用于PET顯像劑生產(chǎn)的常規(guī)合成儀具有許多不足之處：儀器設(shè)備體積龐大,價格非常昂貴；每次合成得到的PET顯像劑的劑量較多,導致反應(yīng)速率和反應(yīng)效率降低；純化步驟非常復雜；反應(yīng)耗時長,難以實現(xiàn)按時按需生產(chǎn)；難以進行功能擴展,每合成一種PET顯像劑需要一組專用設(shè)備,制約了新顯像劑的研發(fā)工作。 在微流控芯片中進行PET顯像劑的合成具有顯著優(yōu)勢。第一,微流控反應(yīng)合成系統(tǒng)可以操控非常小的反應(yīng)體積,因此反應(yīng)物的相對濃度高,反應(yīng)速率快,從而可以大大降低底物的使用量,降低純化的難度。其次,可以極大的縮短合成時間,真正實現(xiàn)按需生產(chǎn)。第三,能顯著提高反應(yīng)的放化產(chǎn)率。第四,反應(yīng)體系小,降低防護成本,提高安全性。第五,反應(yīng)芯片功能擴展性強,可以充分滿足科研需要。這些特點為快速高效制備短半衰期的11C,13N,18F放射性PET顯像劑化合物提供了一個新的實驗平臺,在生命科學和臨床醫(yī)學方面具有廣闊的應(yīng)用前景。因此,研究微流控條件下合成PET顯像劑具有十分重要的意義。 本論文第一章綜述了近年來微流控芯片中合成PET顯像劑的研究進展。著重介紹了微流控條件下合成最常用的顯像劑2-氟-2-脫氧-D-葡萄糖([18F]FDG),將現(xiàn)有的合成系統(tǒng)分為“流動型”和“模塊式”兩大類,并比較了兩類合成系統(tǒng)的優(yōu)缺點。第二章,研發(fā)了一種復合式微流控脫水芯片,用于完成[18F]FDG合成過程中的脫水步驟。這個芯片由玻璃、PDMS、PC三種材質(zhì)制得,采用不可逆封接方法分別制得玻璃-PDMS液路半芯片、PC-PDMS氣路半芯片,然后依靠外力將兩個半芯片可逆封接形成具有玻璃-PDMS... PDMS-PC結(jié)構(gòu)的全芯片。用此芯片對純水、乙腈與水混合溶液以及實際樣品進行脫水實驗研究,結(jié)果令人滿意,可以用于后續(xù)[18F]FDG顯像劑的合成。
[Abstract]:Imaging agent is the key of PET and nuclear medicine. The conventional synthesizer used in the production of PET imaging agent has many disadvantages: the volume of instrument and equipment is huge and the price is very expensive, the dosage of PET imaging agent is more than that of each synthesis, which results in the decrease of reaction rate and reaction efficiency. The purification process is very complex, the reaction time is long, it is difficult to achieve timely production on demand, and it is difficult to expand the function. Each synthesis of PET imaging agent requires a set of special equipment, which restricts the research and development of new imaging agent. The synthesis of PET imaging agent in microfluidic chip has significant advantages. First, the microfluidic reaction synthesis system can control the very small reaction volume, so the relative concentration of reactants is high and the reaction rate is fast, which can greatly reduce the use of substrate and reduce the difficulty of purification. Second, can greatly shorten the synthesis time, real production on demand. Third, the radiochemical yield of the reaction can be improved significantly. The fourth, the reaction system is small, reduces the protection cost, enhances the safety. Fifth, the reaction-chip has strong expansibility and can fully meet the needs of scientific research. These characteristics provide a new experimental platform for rapid and efficient preparation of 11CX ~ (13) N ~ (+) ~ (18) F radioactive PET imaging agent compounds with short half-life, and have a broad application prospect in life sciences and clinical medicine. Therefore, it is of great significance to study the synthesis of PET imaging agents under microfluidic conditions. In the first chapter of this thesis, the research progress of synthetic PET imaging agents in microfluidic chips is reviewed. The synthesis of the most commonly used imaging agent 2-fluoro-2-deoxy-Dglucose ([18F] FDGN) under microfluidic control is introduced in this paper. The existing synthesis systems are divided into "flow type" and "modular type", and the advantages and disadvantages of the two kinds of synthesis systems are compared. In chapter 2, a composite microfluidic dehydration chip is developed to complete the dehydration process of [18F] FDG. The chip is made of three kinds of glass PDMSPC. Glass PDMS liquid circuit half chip PC-PDMS gas circuit half chip is made by irreversible sealing method, and then the two half chips are capped by external force to form glass PDMSs. Full chip of PDMS-PC structure. The dehydration experiments of pure water, acetonitrile and water mixed solution and real samples were carried out with this chip. The results are satisfactory and can be used for the synthesis of [18F] FDG imaging agent.
【學位授予單位】：浙江大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R914

【共引文獻】

相關(guān)期刊論文 前2條

1 何玉林;王雪梅;包寶亮;吳君;趙周社;;GE TRACERLab FX-FN合成器降低~(18)F-FDG合成成本的研究[J];內(nèi)蒙古醫(yī)學院學報;2012年03期

2 孫傳金;朱虹;方可元;劉兵建;;~(18)氟-脫氧葡萄糖合成失敗原因分析[J];醫(yī)學研究生學報;2013年01期

相關(guān)博士學位論文 前2條

1 周麗娜;18F-FDG microPET-CT用于監(jiān)測裸鼠移植瘤吉非替尼療效的實驗研究[D];北京協(xié)和醫(yī)學院;2012年

2 李友鳳;撞擊流微混合器的結(jié)構(gòu)、性能及其在沉淀過程中的應(yīng)用研究[D];中南大學;2012年

相關(guān)碩士學位論文 前2條

1 黃宇石;微流控環(huán)隙流雙水相蛋白質(zhì)分離和酶促反應(yīng)研究[D];西南交通大學;2013年

2 郭蕾;毛細撞擊流反應(yīng)器微觀混合性能的實驗研究[D];北京化工大學;2013年

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本文編號：1871900