本文選題：吡格列酮 + 一磷酸腺苷活化蛋白激酶�。� 參考：《中國(guó)醫(yī)院藥學(xué)雜志》2015年03期

【摘要】：目的:觀察吡格列酮(Pio)對(duì)高脂喂養(yǎng)大鼠骨骼肌組織脂質(zhì)異位沉積的影響。方法:30只Wistar大鼠平均分為對(duì)照組、胰島素抵抗組和吡格列酮干預(yù)組,對(duì)照組給予基礎(chǔ)飼料,其余2組均給予高脂飼料喂養(yǎng),其中吡格列酮干預(yù)組同時(shí)給予吡格列酮20 mg·kg-1·d-1灌胃,各組均喂養(yǎng)16周后檢測(cè)各組大鼠血游離脂肪酸(FFA)水平及骨骼肌組織中三酰甘油(TG)含量,同時(shí)應(yīng)用蛋白免疫印跡法檢測(cè)骨骼肌組織一磷酸腺苷活化蛋白激酶α(AMPKα)磷酸化水平。結(jié)果:與對(duì)照組比較,胰島素抵抗組大鼠胰島素敏感性顯著降低,血FFA水平及骨骼肌組織TG含量增高,骨骼肌組織中AMPKα磷酸化水平降至對(duì)照組水平的52.9%;與胰島素抵抗組比較,吡格列酮干預(yù)組大鼠胰島素敏感性明顯提高,血FFA水平及骨骼肌組織TG含量顯著下降,骨骼肌組織中AMPKα磷酸化水平顯著提高至對(duì)照組的81.3%。但是與對(duì)照組比較,吡格列酮干預(yù)組大鼠血FFA水平及骨骼肌組織TG含量仍然較高,而且胰島素敏感性及骨骼肌組織中AMPKα磷酸化水平仍然顯著降低。結(jié)論:高脂飲食誘導(dǎo)胰島素抵抗,吡格列酮干預(yù)可以通過(guò)降低血FFA水平及減輕骨骼肌組織脂質(zhì)異位沉積改善其胰島素抵抗。
[Abstract]:Aim: to observe the effect of pioglitazone (pioglitazone) on lipid ectopic deposition in skeletal muscle of high fat fed rats. Methods 30 Wistar rats were divided into three groups: control group, insulin resistance group and pioglitazone intervention group. The control group was given basic diet and the other two groups were fed with high fat diet. Pioglitazone group was given pioglitazone 20 mg kg-1 d-1 intragastrically at the same time. After 16 weeks of feeding, the levels of FFAs in blood and the content of TGin in skeletal muscle were measured, and the phosphorylation of adenosine monophosphate activated protein kinase 偽 (AMPK 偽) in skeletal muscle was detected by Western blot. Results: compared with the control group, the insulin sensitivity of rats in insulin resistance group decreased significantly, the level of serum FFA and TG in skeletal muscle tissue increased, and the level of AMPK 偽 phosphorylation in skeletal muscle tissue decreased to 52.9 the level of control group, and that of insulin resistance group was higher than that of insulin resistance group. In pioglitazone group, insulin sensitivity was significantly increased, serum FFA level and TG content in skeletal muscle were significantly decreased, and AMPK 偽 phosphorylation level in skeletal muscle tissue was significantly increased to 81.3% of control group. However, compared with the control group, the serum FFA level and TG content in skeletal muscle of pioglitazone treated rats were still higher, and the insulin sensitivity and AMPK 偽 phosphorylation level in skeletal muscle tissue were still significantly decreased. Conclusion: high fat diet induces insulin resistance. Pioglitazone intervention can ameliorate insulin resistance by reducing serum FFA level and decreasing lipid ectopic deposition in skeletal muscle.
【作者單位】： 湖北省中山醫(yī)院內(nèi)分泌科;
【分類號(hào)】：R965

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