本文選題：μ-阿片受體 + 鈉欲　； 參考：《南方醫(yī)科大學(xué)學(xué)報》2017年09期

【摘要】：目的探討杏仁中央核參與鈉欲中樞調(diào)控的阿片能機制。方法應(yīng)用大腦插管術(shù)和腦微量注射法給大鼠雙側(cè)杏仁中央核內(nèi)單獨或聯(lián)合注射選擇性μ-阿片受體激動劑DAMGO和選擇性μ-阿片受體拮抗劑CTAP,觀察不同的攝鈉模型大鼠對0.3 mol/L NaCl和水?dāng)z入的影響。結(jié)果在"禁水-不完全補水(WD-PR)"攝鈉模型大鼠,在雙瓶測試中,雙側(cè)杏仁中央核內(nèi)分別注射1、2、4 nmol劑量的DAMGO引起劑量依賴性的0.3 mol/L NaCl和水?dāng)z入增加的效應(yīng),而雙側(cè)杏仁中央核內(nèi)分別注射0.5、1、2 nmol劑量的CTAP則產(chǎn)生劑量依賴性的抑制DAMGO 2 nmol注射到同一部位所引起的0.3 mol/L NaCl和水?dāng)z入增加的效應(yīng);在單瓶測試中,雙側(cè)杏仁中央核內(nèi)注射DAMGO 2 nmol對水?dāng)z入量沒有影響。在"皮下聯(lián)合注射利尿劑呋塞米(FURO)與血管緊張素轉(zhuǎn)換酶抑制劑卡托普利(CAP)"攝鈉模型大鼠,雙側(cè)杏仁中央核內(nèi)注射2 nmol劑量的DAMGO引起0.3 mol/L NaCl和水?dāng)z入增加,而雙側(cè)杏仁中央核內(nèi)注射1 nmol CTAP阻斷μ-阿片受體則降低了DAMGO 2 nmol注射到同一部位所引起的0.3 mol/L NaCl和水?dāng)z入增加的效應(yīng);在單瓶測試中,雙側(cè)杏仁中央核內(nèi)注射DAMGO 2 nmol對水?dāng)z入量沒有影響。結(jié)論杏仁中央核μ-阿片受體介導(dǎo)鈉鹽攝入,參與鈉欲的興奮性調(diào)控;μ-阿片受體阻斷劑有望成為研發(fā)鈉欲抑制劑的靶標(biāo)。
[Abstract]:Objective to investigate the opioid mechanism of central amygdaloid nucleus involved in central regulation of natriuretic desire. Methods brain intubation and microinjection were used to inject selective 渭 opioid receptor agonist (DAMGO) and selective 渭 opioid receptor antagonist (CTAP) into bilateral central amygdaloid nucleus of rats. Different sodium uptake models were observed in rats. The effect on 0. 3 mol/L NaCl and water intake. Results in the rat model of sodium intake by water cutoff and incomplete supplementation of water (WD-PRA), the effects of injection of DAMGO in the central amygdaloid nucleus at the dose of 1g 2 nmol into the central amygdaloid nucleus in a dose-dependent manner and the increase in water intake were observed in a double-bottle test. However, CTAP injected into the central amygdaloid nucleus at a dose of 0.5 ~ 1g ~ 2 nmol in a dose-dependent manner inhibited the increase of 0.3 mol/L NaCl and water intake induced by the injection of DAMGO _ 2 nmol to the same site in a single bottle test. Injection of DAMGO 2 nmol into the central nucleus of amygdala had no effect on water intake. In the model rats treated with "combined subcutaneous injection of furosemide" and captopril, an angiotensin converting enzyme inhibitor, the injection of 2 nmol DAMGO into the central amygdaloid nucleus caused an increase in water intake and 0.3 mol/L NaCl in the bilateral central amygdaloid nucleus. The blocking of 渭 -opioid receptor by injection of 1 nmol CTAP into the central amygdala decreased the increase of 0.3 mol/L NaCl and water intake induced by the injection of DAMGO 2 nmol to the same site. Injection of DAMGO 2 nmol into the central nucleus of amygdala had no effect on water intake. Conclusion the 渭 -opioid receptor in the central amygdala mediates sodium uptake and participates in the excitability regulation of natriuretic desire, and the 渭 -opioid receptor blocker is expected to be a target for the development of natriuretic inhibitors.
【作者單位】： 河南科技大學(xué)醫(yī)學(xué)院生理學(xué)教研室;
【基金】：河南省高等學(xué)校重點科研項目計劃(15A180014)
【分類號】：R965
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本文編號：1845680