本文選題：新型納米銀 + 產(chǎn)超廣譜-內(nèi)酰胺酶肺炎克雷伯菌　； 參考：《中國新藥雜志》2017年22期

【摘要】：目的:探討新型納米銀(n Ag-NPs)對產(chǎn)超廣譜β-內(nèi)酰胺酶(ultrabroad spectrum-lactamases,ESBLs)肺炎克雷伯菌的抑制作用。方法:以ESBLs肺炎克雷伯菌為研究對象,采用涂布法檢測殺菌作用,肉湯微量稀釋法測量最低抑菌濃度(minimal inhibitory concentration,MIC),電鏡法n Ag-NPs對ESBLs肺炎克雷伯菌的作用機制。結果:n Ag-NPs(≥0.5 g·m L-1)對ESBLs肺炎克雷伯菌具有100%殺菌作用;0.05 g·m L-1的n Ag-NPs對ESBLs的肺炎克雷伯菌也具有明顯的殺菌作用;5 g·m L-1的n Ag-NPs對ESBLs肺炎克雷伯菌作用5,15,30和60 min,均有明顯的殺菌效果;n Ag-NPs對ESBLs肺炎克雷伯菌的MIC為3.12 g·m L-1。n AgNPs對ESBLs肺炎克雷伯菌的形態(tài)學有明顯影響。結論:n Ag-NPs對ESBLs肺炎克雷伯菌具有明顯的抑制作用,這些實驗結果將為n Ag-NPs的深入研究和臨床應用提供實驗依據(jù)。
[Abstract]:Aim: to investigate the inhibitory effect of a new type of nano-silver (Ag-NPs) on Klebsiella pneumoniae producing extended-spectrum 尾 -lactamases (ESBLs). Methods: the bactericidal effect of Klebsiella pneumoniae (ESBLs) was detected by coating method, the minimal inhibitory concentration was measured by broth dilution method, and the mechanism of the effect of Ag-NPs on Klebsiella pneumoniae was studied by electron microscope. Results 1: n Ag-NPs (鈮，

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