發(fā)布時間：2018-04-14 15:13

  本文選題：紅樹林真菌 + 天然產(chǎn)物��； 參考：《有機(jī)化學(xué)》2015年09期

【摘要】：為獲得活性微生物代謝產(chǎn)物,從海南文昌紅樹林根泥樣品中分離篩選到耐酸真菌Aspergillus sp.OUCMDZ-1914,通過酸性條件發(fā)酵培養(yǎng),發(fā)酵產(chǎn)物經(jīng)過硅膠柱色譜、凝膠柱色譜和半制備高效液相色譜進(jìn)行分離純化,運(yùn)用NMR、MS、UV、IR、X射線單晶衍射等手段鑒定了9個化合物的化學(xué)結(jié)構(gòu),即:(2Z,3E,5E,7E,9E)-4,10-二甲基-11-[3-(2,5-二氧亞基四氫吡咯)基]-2-(2-羥基乙亞基)-11-氧亞基-3,5,7,9-十一碳四烯酸甲酯(1)、RKB-3384A(2)、JBIR-74(3)、JBIR-75(4)、secalonic acid D(5)、oxaline(6)、meleagrin(7)、okaramine H(8)、citreorosein(9),其中1為新化合物.活性測試表明化合物2對甲型流感病毒H1N1的抑制活性與陽性藥利巴韋林相當(dāng),IC50為116.2μmol/L;化合物5對A549、MCF-7和K562細(xì)胞株表現(xiàn)出較好的抑制活性,IC50分別0.23,4.45和3.68μmol/L;化合物6具有α-糖苷酶抑制活性,IC50為0.84mmol/L,與陽性藥阿卡波糖相當(dāng).
[Abstract]:In order to obtain the active microbial metabolites, the acid-resistant fungus Aspergillus sp. OUCMDZ-1914 was isolated from the root mud samples of mangroves in Wenchang, Hainan, and cultured under acidic conditions. The fermentation products were separated by silica gel column chromatography.Gel column chromatography and semi-preparative high performance liquid chromatography were used to separate and purify the chemical structures of 9 compounds.鍗，

本文編號：1749856