本文選題：超高效液相色譜-四級桿-飛行時間質(zhì)譜(UPLC-Q-TOF-MS) + 銀杏磷脂軟膠囊��； 參考：《中成藥》2015年02期

【摘要】：目的建立了銀杏磷脂軟膠囊中12種黃酮類化合物的超高效液相色譜-四級桿-飛行時間質(zhì)譜法(UPLC-Q-TOFMS)快速篩查方法。方法銀杏磷脂軟膠囊甲醇提取物分析以Agilent Poroshell 120 EC-C18(2.1 mm×100 mm,2.7μm)為色譜柱;以乙腈(A)-0.1%甲酸溶液(B)為流動相進行梯度洗脫;體積流量0.2 m L/min;質(zhì)譜定性采用飛行時間質(zhì)譜,負離子模式掃描。結(jié)果在優(yōu)化的液質(zhì)聯(lián)用條件下,通過對照品、數(shù)據(jù)庫匹配及文獻數(shù)據(jù),鑒定出銀杏磷脂軟膠囊中12個黃酮類化合物為(1)3-O-[6-O-(α-L-rhamnosyl)-β-D-glucosyl]-3'-methymyricetin,(2)rhamnosyl-glucosyl methymyricetin,(3)quercetin 3-O-[2-O,6-O bis(α-L-rhamnosyl)-β-D-glucoside],(4)quercetin 3-O-[6-O-(α-L-rhamnosyl)-β-D-glucoside],(5)quercetin 3-O-[2-O-(β-D-quercetin 3-O-[2-O-(β-D-glucosyl)-α-L-rhamnoside],(6)Kaempferol 3-O-[6-O-(α-L-rhamnosyl)-β-D-glucoside],(7)rhamnosyl-glucosyl-apigenin,(8)apigenin7-O-(β-D-glucosyl)(9)槲皮素(quercetin)、(10)芹菜素(apigenin)、(11)山柰酚(kaempferol)及(12)異鼠李素(isorhamnetin)。結(jié)論該方法可作為測定銀杏磷脂軟膠囊總黃酮的手段。
[Abstract]:Objective to establish a rapid screening method for 12 flavonoids in Ginkgo biloba soft capsules by UPLC-Q-TOFMS.Methods the methanol extract of Ginkgo biloba soft capsule was analyzed on Agilent Poroshell 120 EC-C18(2.1 mm 脳 100mm ~ (2.7 渭 m) column, acetonitrile (A) -0.1% formic acid solution (B) was used as mobile phase, volume flow rate was 0.2 mL / min, mass spectrometry was used for qualitative analysis by time-of-flight mass spectrometry (TF-MS), and negative ion mode scanning was used.Results under the optimized conditions of liquid-mass spectrometry, reference substance, database matching and literature data were used.閴村畾鍑洪摱鏉忕７鑴傝蔣鑳跺泭涓，

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