本文選題：超高效液相色譜-質(zhì)譜 + 代謝組學(xué)　； 參考：《色譜》2015年07期

【摘要】：藥物引起的肝損傷是全世界關(guān)注的健康問題,藥源性肝損傷的早期診斷仍然是臨床治療肝損傷的一大難題。本研究中,首先利用對(duì)乙酰氨基酚、四氯化碳、大黃素、雷公藤甲素和馬兜鈴酸構(gòu)建不同類型的肝細(xì)胞損傷模型,利用超高效液相色譜-質(zhì)譜聯(lián)用技術(shù)(UPLC-MS)分別得到正常組和損傷組的細(xì)胞代謝輪廓譜。然后利用模式識(shí)別構(gòu)建分類模型,篩選肝損傷相關(guān)的差異代謝物。結(jié)果顯示,不同類型肝損傷在肝細(xì)胞代謝譜上可以被區(qū)分開,最終鑒定出14種差異代謝物。損傷組肝細(xì)胞經(jīng)保肝陽性藥聯(lián)苯雙酯干預(yù)后,差異代謝物水平均趨向于正常組,在一定程度上驗(yàn)證了差異標(biāo)志物與肝損傷的相關(guān)性。實(shí)驗(yàn)結(jié)果表明細(xì)胞代謝組學(xué)是研究藥物肝損傷的有效工具之一。
[Abstract]:Drug induced liver injury is a health problem in the world. The early diagnosis of drug-induced liver injury is still a difficult problem in clinical treatment of liver injury.In this study, paracetamol, carbon tetrachloride, emodin, triptolide and aristolochic acid were used to construct different types of hepatocyte injury models.UPLC-MS was used to obtain the profiles of cell metabolism in the normal group and the injured group, respectively.Then the classification model was constructed by pattern recognition to screen the differentially metabolites related to liver injury.The results showed that different types of liver injury could be distinguished on the spectrum of hepatic cell metabolism, and 14 different metabolites were identified.The level of different metabolites of liver cells in the injured group was similar to that in the normal group after the intervention of biphenyl diester, a positive drug for liver protection. To a certain extent, the correlation between the differential markers and liver injury was verified.The results show that cell metabolomics is one of the effective tools for the study of drug liver injury.
【作者單位】： 中國科學(xué)院大連化學(xué)物理研究所中國科學(xué)院分離分析化學(xué)重點(diǎn)實(shí)驗(yàn)室;大連醫(yī)科大學(xué)中西醫(yī)結(jié)合學(xué)院(研究院);北京中醫(yī)藥大學(xué);
【基金】：國家科技重大專項(xiàng)(2014ZX09304307-001-006)
【分類號(hào)】：R96;O657.63

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相關(guān)期刊論文 前5條

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本文編號(hào)：1732670