本文選題：那西肽 + 表面活性劑　； 參考：《中國(guó)抗生素雜志》2015年05期

【摘要】：目的研究Triton X-100、Ca Cl2、DMSO、山梨醇、SDS、Tween-80、CTAB等表面活性劑對(duì)活躍鏈霉菌發(fā)酵產(chǎn)那西肽的影響。方法采用單因素實(shí)驗(yàn)研究活躍鏈霉菌的基礎(chǔ)代謝以及表面活性劑加入時(shí)間對(duì)那西肽效價(jià)的影響;采用響應(yīng)面法對(duì)表面活性劑添加濃度進(jìn)行優(yōu)化。結(jié)果基礎(chǔ)代謝研究表明,活躍鏈霉菌產(chǎn)那西肽合成相對(duì)滯后于菌體生長(zhǎng)。多種表面活性劑均顯著影響那西肽的生物合成,整體表現(xiàn)為低濃度促進(jìn)、高濃度抑制。表面活性劑依其類(lèi)型及加入時(shí)機(jī)的不同,對(duì)那西肽產(chǎn)量的影響表現(xiàn)出組間類(lèi)型差異性及組內(nèi)時(shí)間依賴(lài)性。結(jié)論 DMSO、Ca Cl2、Triton X-100在低濃度范圍內(nèi)對(duì)那西肽產(chǎn)量的促進(jìn)效果最為顯著;分別在24、24和48h以DMSO 0.808mg/m L、Ca Cl2 0.799mg/m L和Triton X-100 0.574mg/m L的濃度為最佳添加濃度,該條件下那西肽效價(jià)達(dá)到931.493μg/m L,比優(yōu)化前提高了7.73%。
[Abstract]:Methods the basic metabolism of Streptomyces activis and the effect of surfactant addition time on the titer of natritin were studied by single factor experiment, and the concentration of surfactant was optimized by response surface method.Results the basic metabolism study showed that the synthesis of nacetin produced by Streptomyces activis lagged behind the growth of mycelium.Many surfactants significantly affected the biosynthesis of natritin, which was promoted by low concentration and inhibited by high concentration.The effect of surfactants on the yield of natritin was different among groups and time-dependent according to the type and timing of addition of surfactants.Conclusion DMSO 0.808mg/m Ca Cl2 0.799mg/m L and Triton X-100 0.574mg/m L were the most effective in promoting the yield of natritin in the low concentration range, and the optimal concentrations were DMSO 0.808mg/m Ca Cl2 0.799mg/m L and Triton X-100 0.574mg/m L at 24 h and 48 h, respectively.The titer of nazeptide reached 931.493 渭 g / mL, which was 7.73% higher than that before optimization.
【作者單位】： 安徽工程大學(xué)生物與化學(xué)工程學(xué)院;
【基金】：安徽省高校自然科學(xué)基金(No.KJ2013A048) 國(guó)家級(jí)大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(No.201210363035);國(guó)家級(jí)大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(No.201410363070) 科技型中小企業(yè)技術(shù)創(chuàng)新基金(No.12C26213403295) 安徽省大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃(No.AH201310363143)
【分類(lèi)號(hào)】：R978.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 溫琦;劉登如;陳堅(jiān);堵國(guó)成;;添加表面活性劑促進(jìn)獸疫鏈球菌高產(chǎn)透明質(zhì)酸[J];化工進(jìn)展;2006年09期

2 周s，

本文編號(hào)：1732631