本文選題：銀杏　切入點：內(nèi)生真菌　出處：《江蘇農(nóng)業(yè)科學(xué)》2017年13期

【摘要】：對前期研究發(fā)現(xiàn)的1株分離自銀杏樹皮組織的具有良好的抗腫瘤活性內(nèi)生真菌米曲霉菌(Aspergillus oryzae)YX-5菌株的發(fā)酵條件及活性產(chǎn)物穩(wěn)定性進行研究。以發(fā)酵后的菌體干質(zhì)量、粗提物質(zhì)量和粗提物抗腫瘤活性為指標(biāo),對YX-5菌株的發(fā)酵溫度、pH值、轉(zhuǎn)速和發(fā)酵時間等條件進行優(yōu)化。結(jié)果表明,最適宜的發(fā)酵條件為溫度25℃、pH值7.0、搖床轉(zhuǎn)速110 r/min、發(fā)酵時間7 d。以粗提物的抗腫瘤活性為指標(biāo),比較發(fā)酵液粗提物經(jīng)熱處理、酸堿處理、紫外照射和蛋白酶處理后的穩(wěn)定性,發(fā)現(xiàn)YX-5菌株的活性產(chǎn)物在常溫、中性和酸性環(huán)境中穩(wěn)定性較好,對紫外線和蛋白酶有較強的抗性,但在超過100℃高溫條件下或pH值超過9.0的堿性環(huán)境中穩(wěn)定性較差。該菌株粗提物在自然條件下總體穩(wěn)定性較高,不易發(fā)生變化,有利于活性成分分離工作的開展。
[Abstract]:The fermentation conditions and the stability of the active product of an endophytic fungus Aspergillus oryzae oryzae)YX-5, which was isolated from the bark of Ginkgo, were studied. The quality of crude extract and the antitumor activity of crude extract were used as indexes to optimize the fermentation temperature, pH value, rotational speed and fermentation time of YX-5 strain. The optimum fermentation conditions were as follows: temperature 25 鈩，

本文編號：1681897