發(fā)布時(shí)間：2018-03-25 21:24

  本文選題：氨甲環(huán)酸　切入點(diǎn)：二維高效液相色譜　出處：《中國(guó)醫(yī)院藥學(xué)雜志》2016年23期

【摘要】：目的:建立全自動(dòng)二維色譜法(2D-LC-UV)測(cè)定血漿中氨甲環(huán)酸濃度的方法,用于臨床上行關(guān)節(jié)置換術(shù)患者圍術(shù)期氨甲環(huán)酸治療藥物濃度的監(jiān)測(cè)。方法:待測(cè)物在一維柱ASTON SPX(100 mm×4.6 mm,5μm)上初步分離,通過(guò)中間柱ASTO N SHC(10 mm×4.6 mm,3μm)截取保留并在二維柱ASTON SX1(150 mm×4.6 mm,5μm)上進(jìn)一步分離,200μL樣品進(jìn)樣,并最終測(cè)定。流速均為1.0mL·min~(-1),柱溫為40℃,紫外檢測(cè)波長(zhǎng)為220 nm。所建立方法運(yùn)用于58名行膝關(guān)節(jié)/髖關(guān)節(jié)置換術(shù)的患者圍術(shù)期中氨甲環(huán)酸血漿樣本的檢測(cè)分析。結(jié)果:在所建立的色譜條件下,氨甲環(huán)酸與各雜質(zhì)分離良好,線性范圍為5~300μg·mL~(-1),最低檢測(cè)限為5μg·mL~(-1),批間批間精密度均小于6.6%,提取回收率大于72.4%。58名患者圍術(shù)期中氨甲環(huán)酸首次給藥后3個(gè)時(shí)間點(diǎn)(5 min,30 min和2 h)平均血漿濃度分別為:(103.3±20.4)μg·mL~(-1),(53.0±14.8)μg·mL~(-1)和(22.8±8.3)μg·mL~(-1)。手術(shù)中男女之間氨甲環(huán)酸血藥濃度無(wú)顯著性差異(P0.05),而不同手術(shù)種類之間(膝關(guān)節(jié)/髖關(guān)節(jié)置換術(shù))氨甲環(huán)酸血藥濃度存在顯著性差異(P0.05)。結(jié)論:此法操作簡(jiǎn)單,準(zhǔn)確、精密度好,適用于臨床氨甲環(huán)酸血漿濃度的監(jiān)測(cè)及濃度-療效關(guān)系研究。
[Abstract]:Objective: to establish a method for the determination of carbamate in plasma by automatic two-dimensional chromatography (2D-LC-UV). To monitor the perioperative concentration of methionine in clinical patients undergoing arthroplasty. Methods: the substance to be tested was preliminarily separated on one dimensional column ASTON SPX(100 mm 脳 4. 6 mm ~ 5 渭 m. ASTO N SHC(10 mm 脳 4. 6 mm ~ (3 渭 m) was used to intercept and retain the sample and to separate the sample on a two dimensional column (ASTON SX1(150 mm 脳 4. 6 mm ~ 5 渭 m). The results were as follows: the flow rate was 1.0mL min-1n, the column temperature was 40 鈩，

本文編號(hào)：1664934