本文選題：N-雙嘧啶取代芳胺衍生物　切入點(diǎn)：雙氧水　出處：《化學(xué)試劑》2017年10期

【摘要】：報(bào)道了一種N-雙嘧啶取代芳胺衍生物2-(3-氯苯基)-N-(2-(3-氯苯基)-6-甲基嘧啶-4-基)-N-(2-甲氧苯基)-6-甲基嘧啶-4-胺的合成、表征及其抗氧化生物活性,該N-雙嘧啶取代芳胺衍生物是以3-氯芐腈為原料經(jīng)4步反應(yīng)合成而得到,其結(jié)構(gòu)通過1HNMR、13CNMR、HR-MS進(jìn)行了表征和確認(rèn)�；衔锏目寡趸饔门c細(xì)胞毒作用分別通過是否進(jìn)行雙氧水處理的SH-SY5Y細(xì)胞與CCK-8試劑盒進(jìn)行檢測,通過熒光探針DCFH-DA標(biāo)記檢測細(xì)胞內(nèi)ROS含量。結(jié)果表明,該化合物具有抗雙氧水氧化損傷的可能,能夠顯著提高細(xì)胞存活率且沒有明顯的細(xì)胞毒性。
[Abstract]:This paper reports the synthesis, characterization and antioxidant biological activity of a derivative of N-dipyrimidine substituted aryl amines (2-O3-chlorophenyl) -N-n- (3-chlorophenyl) -6-methylpyrimidine (4-methylpyrimidine) -6-methoxyphenyl (2-methoxyphenyl) -6-methylpyrimidine-4- (4-amine), which is a derivative of N-dipyrimidine substituted aryl amines. The N-dipyrimidine substituted aromatic amine derivative was synthesized from 3-chlorobenzylonitrile in four steps. Its structure was characterized and confirmed by 1H-NMR-13CNMR-HR-MS. The antioxidation and cytotoxicity of the compounds were detected by SH-SY5Y cells treated with hydrogen peroxide and CCK-8 kit, respectively. Fluorescence probe DCFH-DA labeling was used to detect the content of ROS in the cells. The results showed that the compound had the possibility of resisting oxidative damage of hydrogen peroxide and could significantly improve the cell survival rate without obvious cytotoxicity.
【作者單位】： 武漢科技大學(xué)化學(xué)與化工學(xué)院;武漢科技大學(xué)生物醫(yī)學(xué)研究院;
【基金】：湖北省自然科學(xué)基金資助項(xiàng)目(2015CFB490)
【分類號】：R914
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本文編號：1654474