本文選題：COX-抑制劑　切入點(diǎn)：體外培養(yǎng)　出處：《臨床合理用藥雜志》2014年14期

【摘要】：目的觀察塞來(lái)昔布體外實(shí)驗(yàn)效應(yīng)及相關(guān)影響因素,探索其體外藥物作用的相關(guān)機(jī)制。方法利用MTT比色法測(cè)定塞來(lái)昔布對(duì)多種細(xì)胞系、藥物作用不同時(shí)間以及與細(xì)胞短時(shí)間接觸后產(chǎn)生的抑制率;通過(guò)測(cè)定血紅蛋白的吸光度來(lái)反應(yīng)不同條件下藥物引起的溶血作用;觀察不同培養(yǎng)基血清濃度、藥物配置后放置不同時(shí)間、超聲及濾膜過(guò)濾等物理處理方式對(duì)藥物體外作用的影響。結(jié)果體外培養(yǎng)基條件下,塞來(lái)昔布可同時(shí)抑制腫瘤細(xì)胞系及正常細(xì)胞系的生長(zhǎng),藥物的這種作用在與藥物短暫接觸后即會(huì)產(chǎn)生,且藥物抑制作用會(huì)隨作用時(shí)間的延長(zhǎng)而逐漸增強(qiáng);較高濃度藥物可在短時(shí)間內(nèi)引起紅細(xì)胞胞膜破裂,這種作用可在4℃的環(huán)境下中止,恢復(fù)溫度后紅細(xì)胞再次破裂;培養(yǎng)基中血清濃度、藥物配置后放置時(shí)間以及超聲和濾膜過(guò)濾等因素均可明顯影響藥物的體外抑制作用。結(jié)論培養(yǎng)基中未溶解的游離形態(tài)藥物是塞來(lái)昔布體外效應(yīng)的作用主體,其通過(guò)快速黏附于細(xì)胞膜系統(tǒng)而產(chǎn)生一系列特殊的實(shí)驗(yàn)現(xiàn)象,故可影響藥物游離形式的方式均可明顯影響藥物的體外效應(yīng)。
[Abstract]:Objective to observe the experimental effect of celecoxib in vitro and its related influencing factors, and to explore the mechanism of its drug action in vitro. Methods the effects of celecoxib on various cell lines were determined by MTT colorimetry. Inhibition rate of drugs at different time and short contact with cells; hemolysis induced by drugs under different conditions by measuring the absorbance of hemoglobin; observation of serum concentration in different media, The effects of ultrasound and filter membrane filtration on the drug action in vitro were studied. Results Celecoxib could inhibit the growth of tumor cell line and normal cell line at the same time. This effect of the drug will be produced after brief contact with the drug, and the inhibitory effect of the drug will gradually increase with the prolongation of the action time, and the higher concentration of the drug can cause the rupture of the erythrocyte membrane in a short time. This effect can be stopped at 4 鈩，

本文編號(hào)：1653094