發(fā)布時(shí)間：2018-03-19 22:45

  本文選題：MK-　切入點(diǎn)：自發(fā)運(yùn)動(dòng)　出處：《復(fù)旦學(xué)報(bào)(醫(yī)學(xué)版)》2015年03期 　論文類型：期刊論文

【摘要】：目的在不同光照條件下,地卓西平馬來(lái)酸鹽(dizocilpine,MK-801)急性處理對(duì)斑馬魚幼魚自發(fā)運(yùn)動(dòng)和焦慮行為的影響。方法野生型AB品系斑馬魚幼魚(5天和7天)暴露在不同濃度MK-801(0、10、20、50、100、200μmol/L)溶液中。在光照與黑暗交替條件下,記錄斑馬魚幼魚每分鐘游動(dòng)距離和趨觸性。結(jié)果光照條件下,MK-801急性處理能顯著降低幼魚自發(fā)運(yùn)動(dòng)(P0.05);黑暗條件下,MK-801急性處理也能降低幼魚自發(fā)運(yùn)動(dòng)(P0.05),但10μmol/L MK-801對(duì)5天幼魚自發(fā)運(yùn)動(dòng)無(wú)顯著影響(P0.05);黑暗條件下,7天幼魚在100μmol/L MK-801作用下抗焦慮能力顯著增加,5天幼魚在20、50μmol/L MK-801作用下有顯著的抗焦慮能力(P0.05);光照條件下,斑馬魚幼魚的抗焦慮行為都無(wú)顯著變化(P0.05)。結(jié)論 MK-801誘導(dǎo)幼魚自發(fā)運(yùn)動(dòng)降低;在黑暗條件下,MK-801用藥組(20、50、100和200μmol/L)的幼魚表現(xiàn)出抗焦慮行為;年齡、藥物劑量和光照條件共同作用影響斑馬魚幼魚的行為模式。
[Abstract]:Objective under different illumination conditions, The effect of dizocilpine maleate on spontaneous movement and anxiety behavior of juvenile zebrafish was studied. Methods Wild AB strain of juvenile zebrafish was exposed to MK-801 (102050U / L) solution for 5 days and 7 days. Under the alternating conditions of light and darkness, the effect of dizocilpine on the spontaneous movement and anxiety behavior of juvenile zebrafish was studied. The swimming distance and thixotropism per minute of juvenile zebrafish were recorded. Results under light condition, acute treatment with MK-801 significantly reduced the spontaneous movement of young fish P0.05U, and acute treatment with MK-801 also decreased the spontaneous movement of juvenile fish P0.05, but 10 渭 mol/L MK-801 for 5 days. There was no significant effect on the spontaneous movement of young fish (P0.05), but the ability of anti-anxiety was significantly increased under 100 渭 mol/L MK-801 for 7 days under dark conditions, and the ability of anti-anxiety was significantly increased under the action of 2050 渭 mol/L MK-801 at 20 渭 mol/L MK-801 for 5 days. There was no significant change in the anti-anxiety behavior of juvenile zebrafish (P0.050.Conclusion MK-801 induces the decrease of spontaneous movement of juvenile fish, and the juvenile of MK-801 drug group shows anti-anxiety behavior under dark conditions, which is 200 渭 mol / L and 20 渭 mol / L). The effects of drug dosage and light condition on the behavior patterns of juvenile zebrafish were studied.
【作者單位】： 復(fù)旦大學(xué)附屬兒科醫(yī)院兒科研究所;復(fù)旦大學(xué)兒童發(fā)育與疾病轉(zhuǎn)化醫(yī)學(xué)研究中心-上海市出生缺陷防治重點(diǎn)實(shí)驗(yàn)室;上海中醫(yī)藥大學(xué)中醫(yī)方證與系統(tǒng)生物學(xué)研究中心;
【基金】：國(guó)家自然科學(xué)基金(81271509)~~
【分類號(hào)】：R965

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本文編號(hào)：1636353