本文選題：血管性癡呆　切入點(diǎn)：重組人生長(zhǎng)激素　出處：《山東醫(yī)藥》2017年47期 　論文類型：期刊論文

【摘要】：目的觀察重組人生長(zhǎng)激素(rh GH)對(duì)血管性癡呆(VD)大鼠學(xué)習(xí)記憶能力、空間探索能力的影響,并探討其可能機(jī)制。方法 45只大鼠隨機(jī)分為實(shí)驗(yàn)組、模型組、正常組各15只,其中實(shí)驗(yàn)組和模型組通過(guò)不同時(shí)間點(diǎn)雙側(cè)頸總動(dòng)脈結(jié)扎法(結(jié)扎左側(cè)頸總動(dòng)脈3 d后再結(jié)扎右側(cè)頸總動(dòng)脈)制備VD模型,正常組用同樣方法分離雙側(cè)頸總動(dòng)脈,但不結(jié)扎頸總動(dòng)脈;造模后第1天開始,實(shí)驗(yàn)組大鼠頸部皮下注射rh GH(0.2 IU/100 g),正常組和模型組給予等量生理鹽水,1次/d,連續(xù)28 d。采用水迷宮實(shí)驗(yàn)評(píng)價(jià)大鼠學(xué)習(xí)記憶能力及空間探索能力,ELISA法檢測(cè)血清、皮質(zhì)、海馬中血管內(nèi)皮生長(zhǎng)因子(VEGF)、胰島素樣生長(zhǎng)因子1(IGF-1)、生長(zhǎng)激素(GH),TUNEL凋亡染色觀察神經(jīng)元凋亡情況[以積分光密度值(IOD值)表示]。結(jié)果與正常組比較,實(shí)驗(yàn)組第1~4天及模型組第1~6天逃避潛伏期長(zhǎng)(P均0.05);與模型組比較,實(shí)驗(yàn)組第2、4、5、6天逃避潛伏期短(P均0.05)。與模型組比較,實(shí)驗(yàn)組、正常組經(jīng)過(guò)平臺(tái)次數(shù)多(P均0.05)。與模型組比較,實(shí)驗(yàn)組血清、皮質(zhì)、海馬中VEGF、IGF-1、GH水平升高(P均0.05);與正常組比較,模型組血清、皮質(zhì)、海馬中VEGF、IGF-1、GH水平降低(P均0.05)。與模型組比較,實(shí)驗(yàn)組、正常組海馬TUNEL凋亡染色I(xiàn)OD值降低(P均0.05)。結(jié)論 rh GH可改善VD大鼠學(xué)習(xí)記憶能力、空間探索能力,其機(jī)制可能與其可增加血清、皮質(zhì)、海馬中VEGF和IGF-1水平有關(guān)。
[Abstract]:Objective To observe the effects of recombinant human growth hormone (RH GH) on vascular dementia (VD) learning and memory ability of rats, effects of space exploration, and to explore its possible mechanism. Methods 45 rats were randomly divided into experimental group, model group, normal group with 15 rats in each group, the experimental group and model group through different at the time of bilateral common carotid artery occlusion (ligation of the left carotid artery 3 d after ligation of right common carotid artery) preparation of VD model, the normal group with the same method, separation of bilateral carotid artery without ligation of common carotid artery; the first day after modeling, the subcutaneous injection of RH rats (GH group 0.2 IU/100 g), the normal group and model group were given normal saline, 1 times /d, for 28 d. by using water maze test to evaluate the learning and memory ability of rats and space exploration, detection of serum ELISA, cortex, vascular endothelial growth factor in hippocampus (VEGF), insulin-like growth factor 1 (IGF-1) students. 闀挎縺绱，

本文編號(hào)：1621130