本文選題：林肯鏈霉菌　切入點(diǎn)：三重復(fù)合誘變　出處：《中國(guó)抗生素雜志》2017年08期 　論文類型：期刊論文

【摘要】：為進(jìn)一步提高林可霉素生產(chǎn)發(fā)酵水平,本文以林肯鏈霉菌LK15-35為出發(fā)菌株,采用氯化鋰(Li Cl)、常壓室溫等離子體(ARTP)和紫外線(UV)三重復(fù)合誘變,首先用Li Cl預(yù)處理4h,然后用ARTP照射60s,再用UV照射30s,最終獲得高產(chǎn)突變株LK16-77。結(jié)果表明,搖瓶發(fā)酵單位提高30.0%,經(jīng)180噸罐生產(chǎn)驗(yàn)證,平均放罐效價(jià)提高21.4%。穩(wěn)定性傳代驗(yàn)證證明該突變株高產(chǎn)性能遺傳穩(wěn)定。
[Abstract]:In order to further improve the fermentation level of lincomycin, Streptomyces Lincoln LK15-35 was used as the starting strain. The high yield mutant LK16-77 was obtained by pretreatment with Li Cl for 4 h, then irradiated with ARTP for 60 s and then irradiated with UV for 30 s. The results showed that the fermentation unit of shaking flask was increased by 30.0% and verified by 180 ton pot production. The average titer of the mutant was increased by 21.4.The stability test proved that the mutant was genetically stable in high yield.
【作者單位】： 寧夏泰瑞制藥股份有限公司;
【分類號(hào)】：R915
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本文編號(hào)：1616994