本文選題：小鼠　切入點：衰老　出處：《安徽醫(yī)科大學》2014年碩士論文　論文類型：學位論文

【摘要】：背景 隨著國內人口老齡化的加速，如何延緩衰老及衰老相關的認知功能下降從而改善老年人生活質量已成為研究的熱點。Synaptotagmin (Syt) Ⅰ屬于突觸前囊泡蛋白，參與調控突觸囊泡同步的快速胞吐過程。當前的研究顯示老年快速老化小鼠（SAMP8）背側海馬SytⅠ蛋白升高，并且和年齡相關的空間學習和記憶障礙相關。膠質原纖維酸性蛋白(Glial Fibrillary Acidic Protein，GFAP)是星形膠質細胞中間絲的主要蛋白，是其特異性生物標志物，它的表達隨著年齡的增長而升高。大量的研究表明，星形膠質細胞和學習、記憶及認知功能，特別是各種年齡相關的神經變性疾病，，如阿爾茨海默氏病、帕金森病密切相關。阿卡波糖作為α-糖苷酶抑制劑，長期口服具有穩(wěn)定餐后及空腹血糖、抑制晚期糖基化終末產物生成的作用。它也有預防和延緩衰老相關疾病如2型糖尿病及心血管疾病的發(fā)生發(fā)展作用。這些提示阿卡波糖可能具有延緩衰老效應。 目的 探討長期口服阿卡波糖對老年SAMP8小鼠背海馬SytⅠ和GFAP蛋白含量的影響。 方法 實驗動物先隨機分成老年空白對照組和阿卡波糖組。所有小鼠自由飲食。阿卡波糖組小鼠從3月齡開始隨水給予阿卡波糖，至9月齡時再加入一批3月齡青年組小鼠作為青年組，與先前兩組小鼠同時進行行為學任務檢測，選用免疫組化技術檢測3組SAMP8小鼠背海馬各區(qū)中Syt Ⅰ和GFAP表達。 結果 ①所有受試小鼠背海馬齒狀回、CA1區(qū)及CA3區(qū)內Syt Ⅰ和GFAP蛋白均有表達；②與青年對照組相比，老年對照組小鼠齒狀回顆粒細胞層和分子層、CA1區(qū)及CA3區(qū)各層SytⅠ相對含量均顯著升高（Ps＜0.05）；阿卡波糖處理組小鼠齒狀回顆粒細胞層、分子層以及CA1和CA3區(qū)各層的SytⅠ相對含量均顯著低于同齡對照組（Ps＜0.05）；③與青年對照組相比，老年對照組小鼠齒狀回、CA1區(qū)及CA3區(qū)GFAP相對含量均顯著升高（Ps＜0.05）；阿卡波糖處理組小鼠CA1區(qū)和 CA3區(qū)的GFAP相對含量均顯著低于同齡對照組（Ps＜0.05）。 結論①在SAMP8小鼠背海馬各區(qū)亞層內，SytⅠ和GFAP含量呈不同程度的年齡相關性增加；②長期口服阿卡波糖可以減輕SAMP8小鼠其背海馬各亞層內SytⅠ和GFAP的衰老效應。
[Abstract]:Background. With the acceleration of the aging population in China, how to delay senescence and the decline of cognitive function related to aging and improve the quality of life of the elderly has become a hot topic. Synaptotagmin SytI belongs to the presynaptic vesicle protein. It is involved in the regulation of rapid exocytosis in synaptic vesicle synchronization. Recent studies have shown that the Syt I protein in the dorsal hippocampus of aged mice with rapid aging is increased. The glial fibrillary acidic protein (Glial Fibrillary Acidic protein GFAP) is the main protein of astrocytic mesenchyme and a specific biomarker. A large number of studies have shown that astrocytes and learning, memory and cognitive functions, especially age-related neurodegenerative diseases, such as Alzheimer's disease, increase with age. Parkinson's disease is closely related. As an inhibitor of 偽 -glucosidase, acarbose has stable postprandial and fasting blood glucose for a long time. It can also prevent and delay the occurrence and development of aging related diseases such as type 2 diabetes and cardiovascular diseases. These results suggest that acarbose may have the effect of delaying senescence. Purpose. To investigate the effects of long-term oral acarbose on the contents of Syt 鈪

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