本文選題：過(guò)氧化氫酶　切入點(diǎn)：納米脂質(zhì)體　出處：《中國(guó)醫(yī)院藥學(xué)雜志》2015年17期 　論文類型：期刊論文

【摘要】：目的:研究過(guò)氧化氫酶(catalase,CA)/尿酸酶(uricase,UA)復(fù)合納米脂質(zhì)體(catalase and uricase nanoliposome,CULP)中過(guò)氧化氫酶在大鼠體內(nèi)的藥效學(xué)。方法:采用逆向蒸發(fā)法制備了CULP,用激光粒度儀測(cè)定平均粒徑。測(cè)定CULP及CA的最適溫度和最適pH值。大鼠靜脈注射過(guò)氧化氫建立高過(guò)氧化氫大鼠模型,建模后分別靜脈注射CULP和CA,測(cè)定不同時(shí)間點(diǎn)大鼠血清中過(guò)氧化氫的濃度。結(jié)果:CULP的平均粒徑為1 000 nm。CULP和CA最適溫度均為40℃,最適pH值分別為為8.0和7.0。在同一溫度和pH條件下,CULP中CA的活性高于游離CA;且CULP在大鼠體內(nèi)降低過(guò)氧化氫濃度的能力高于游離CA。結(jié)論:CULP在大鼠體內(nèi)降低過(guò)氧化氫水平的能力優(yōu)于游離CA,為過(guò)氧化氫酶的臨床應(yīng)用提供實(shí)驗(yàn)依據(jù)。
[Abstract]:Aim: to study the pharmacodynamics of catalase and uricase nanoliposome CULPs in rats. Methods: CULPs were prepared by reverse evaporation and the average particle size was measured by laser particle size analyzer. The optimal temperature and pH of CULP and CA were determined. The rat model of high hydrogen peroxide was established by intravenous injection of hydrogen peroxide. After modeling, CULP and CA were injected intravenously to determine the concentration of hydrogen peroxide in serum of rats at different time points. Results the mean particle size of 1 000 nm.CULP and the optimum temperature of CA were 40 鈩，

本文編號(hào)：1572318