本文選題：羅蘇伐他汀　切入點(diǎn)：酮還原酶　出處：《西南師范大學(xué)學(xué)報(bào)(自然科學(xué)版)》2015年02期 　論文類(lèi)型：期刊論文

【摘要】：將酮還原酶應(yīng)用于降血脂藥羅蘇伐他汀的制備.通過(guò)高通量篩選,酮還原酶KRED-4可催化羅蘇伐他汀乙酯的制備反應(yīng).經(jīng)過(guò)反應(yīng)條件的初步優(yōu)化后,確定了該酶催化反應(yīng)的最適溫度為25~35℃、最適pH值為6.0、初始底物濃度1g/L和酶濃度2g/L.在最優(yōu)條件下,可將底物1完全轉(zhuǎn)化為產(chǎn)物2羅蘇伐他汀乙酯,產(chǎn)物的構(gòu)型完全正確,且de99.9%,為建立羅蘇伐他汀制備的綠色工藝提供了新的思路.
[Abstract]:Ketone reductase was applied to the preparation of rosuvastatin, a lipid-lowering drug. Through high throughput screening, ketone reductase KRED-4 could catalyze the preparation of rosuvastatin ethyl ester. The optimum reaction temperature, pH value, initial substrate concentration and enzyme concentration were 2535 鈩，

本文編號(hào)：1570588