本文選題：脂多糖　切入點(diǎn)：膿毒癥　出處：《中國藥科大學(xué)學(xué)報(bào)》2015年05期 　論文類型：期刊論文

【摘要】：為探討自噬對(duì)脂多糖(LPS)所致膿毒癥小鼠急性肺損傷的影響,采用健康昆明小鼠隨機(jī)分為空白組、模型組(LPS,10 mg/kg),雷帕霉素組(RAP,6 mg/kg),RAP-LPS組(RAP 6 mg/kg+LPS 10 mg/kg),3-甲基腺嘌呤組(3-MA,300μg/kg)和3-MA-LPS組(3-MA 300μg/kg+LPS 10 mg/kg),腹腔注射,給藥6 h后,檢測小鼠死亡率、肺組織中性粒細(xì)胞浸潤、以及TNF-α、LC3和P62的表達(dá)。結(jié)果顯示在LPS組中,小鼠肺組織中性粒細(xì)胞浸潤顯著增多,死亡率增加,TNF-α、LC3-Ⅱ和P62表達(dá)升高;RAP-LPS組較模型組死亡率下降,中性粒細(xì)胞浸潤、P62和TNF-α均減少,而LC3-Ⅱ略升高;在3-MA-LPS組中,LC3-Ⅱ較LPS組降低,而中性粒細(xì)胞浸潤、P62和TNF-α則沒有差異。結(jié)果表明在LPS所致的膿毒癥中,小鼠肺組織存在自噬流阻斷,自噬體成熟障礙,RAP則能逆轉(zhuǎn)LPS所致的肺組織自噬流障礙,改善細(xì)胞內(nèi)環(huán)境,減輕炎性反應(yīng),降低死亡率。
[Abstract]:To investigate the effect of autophagy on acute lung injury in septic mice induced by lipopolysaccharide (LPS), healthy Kunming mice were randomly divided into blank group. Model group (10 mg / kg), rapamycin group (6 mg / kg), RAP-LPS group (10 mg / kg / kg) and 3-MA-300 渭 g / kg LPS (10 mg / kg) and 3-MA-LPS group (30 渭 g / kg LPS / kg / kg), intraperitoneal injection of rapamycin 6 mg / kg / kg / kg RAP-LPS group, lung neutrophil infiltration, lung tissue neutrophil infiltration, intraperitoneal injection of rapamycin 6 mg / kg / kg RAP-LPS group, lung tissue neutrophil infiltration, intraperitoneal injection of rapamycin 6 mg / kg / kg RAP-LPS group, neutrophil infiltration in lung tissue, The results showed that in LPS group, the neutrophil infiltration was significantly increased, the mortality increased, the expression of TNF- 偽 TNF- 偽 -LC3- 鈪，

本文編號(hào)：1569977