發(fā)布時(shí)間：2018-03-04 18:05

  本文選題：環(huán)丙沙星　切入點(diǎn)：高效液相色譜熒光法　出處：《中國(guó)臨床藥理學(xué)雜志》2016年03期 　論文類型：期刊論文

【摘要】：目的建立測(cè)定人血清中環(huán)丙沙星濃度的高效液相色譜方法。方法血清樣品經(jīng)5%高氯酸沉淀蛋白。色譜柱為Eclipse XDB-C18柱(150 mm×4.6mm,5μm),流動(dòng)相為乙腈-0.025 mol·L~(-1)磷酸溶液(用三乙胺調(diào)p H至3.0)=15∶85,流速為1.0 m L·min~(-1);柱溫為40℃,檢測(cè)波長(zhǎng)為278 nm,加替沙星為內(nèi)標(biāo)。結(jié)果環(huán)丙沙星在0.05~50.00μg·m L~(-1)線性關(guān)系良好(r0.999 5);低、中、高質(zhì)量濃度質(zhì)控樣品(0.1,1.0,4.0μg·m L~(-1))的日內(nèi)相對(duì)標(biāo)準(zhǔn)偏差(RSD)為0.98%~2.63%,日間RSD為1.87%~3.16%,絕對(duì)回收率為49.2%~54.7%。結(jié)論本法有良好的特異性、靈敏度、精密度和準(zhǔn)確度,適用于臨床患者的血藥濃度測(cè)定。
[Abstract]:Objective to establish a high performance liquid chromatography method for the determination of ciprofloxacin in human serum. Methods Serum samples were precipitated by 5% perchloric acid. The chromatographic column was Eclipse XDB-C18 column (150mm 脳 4.6 mm) and the mobile phase was acetonitrile (-0.025 mol 路L ~ (-1)) phosphoric acid solution (adjusted by triethylamine). The flow rate is 1.0 mL 路min ~ (-1) and the column temperature is 40 鈩，

本文編號(hào)：1566722