本文關鍵詞： 熒光猝滅 牛血清白蛋白 乙酰膽堿酯酶 氯沙坦鉀 蘆丁 黃芩苷 氮摻雜碳量子點 檢測汞離子　出處：《河北大學》2017年碩士論文　論文類型：學位論文

【摘要】：本論文主要應用熒光光譜法和紫外-可見光譜法等多種方法探究了藥物與蛋白質的相互作用和通過水熱法合成氮摻雜的碳量子點實現(xiàn)了對Hg2+的測定。本論文分為四章。第一章:歸納概括了血清白蛋白、乙酰膽堿酯酶結構和功能,概述了用光譜法探究蛋白質與藥物相互作用機理和內容。對碳量子點性質,合成方法及在有關領域的使用價值做了概括。第二章:用不同的光譜手段對氯沙坦鉀與牛血清白蛋白間的相互作用進行探究,考察了黃酮化合物蘆丁和黃芩苷存在下對其相互作用的干擾。氯沙坦鉀可以明顯地使牛血清白蛋白的熒光發(fā)生猝滅,證明氯沙坦鉀對牛血清白蛋白的猝滅機理為靜態(tài)猝滅過程。熱力學參數(shù)ΔH(-134.3kJ·mol-1),ΔS(-368.4J.mol-1.K-1)和ΔG(-24.52kJ·mol-1~-20.83kJ·mol-1),表明氯沙坦鉀與牛血清白蛋白之間的主要作用力為范德華力和氫鍵。此外,牛血清白蛋白與氯沙坦鉀間的結合距離r為3.183nm,表明它們之間發(fā)生能量轉移的可能性很大。當在蘆丁和黃芩苷存在的條件下,牛血清白蛋白與氯沙坦鉀的結合距離就變?yōu)?.225nm和3.494nm。此外牛血清白蛋白與氯沙坦鉀的結合常數(shù)降低,說明氯沙坦鉀、蘆丁和黃芩苷在牛血清白蛋白上面存在競爭。最后,通過同步熒光光譜和三維光譜探究了藥物與牛血清白蛋白作用前后,牛血清白蛋白結構的變化。第三章:利用熒光光譜法,紫外-可見吸收光譜法和圓二色譜法探究了黃芩苷與乙酰膽堿酯酶(AChE)的相互作用,得出了它們作用的機理,并對其結合常數(shù)、結合位點數(shù)以及結合距離作了計算,得出黃芩苷明顯地猝滅了乙酰膽堿酯酶的內源性熒光,猝滅的機制主要是靜態(tài)猝滅。熱力學參數(shù)ΔH(-174.6kJ·mol-1),ΔS(-489.9 J·mol-1.K-1)和ΔG(-28.61kJ·mol-1~-23.71kJ·mol-1),說明黃芩苷與乙酰膽堿酯酶間的作用力是范德華力和氫鍵。此外,乙酰膽堿酯酶與黃芩苷的結合距離r為2.85nm,說明它們之間發(fā)生能量轉移的幾率很大。用同步熒光光譜,三維熒光光譜和圓二色譜探究了黃芩苷與乙酰膽堿酯酶作用前后,乙酰膽堿酯酶構象的變化。最后,對AChE的活性進行了研究,得出其IC50為124μM.第四章:通過水熱法,以絲氨酸和乙二胺為原料合成了水溶性良好的氮摻雜的熒光碳量子點。在360nm的最佳激發(fā)波長下該氮摻雜碳量子點的量子產率為8.66%,用透射電鏡、高分辨透射電鏡、紅外光譜和熒光光譜等方法對其進行了系統(tǒng)的分析表征,表明該氮摻雜的碳量子點表面上含有豐富的羥基和羧基等基團,因此其水溶性良好。通過實驗表明,汞離子使該氮摻雜碳量子點的熒光強度明顯降低,其線性范圍為1-20μM,檢出限為7.33×10-5 mol·L-1,說明氮摻雜碳量子點在離子檢測方面具有潛在的應用價值。
[Abstract]:A variety of methods in this thesis using fluorescence spectroscopy and UV Vis spectroscopy to explore the interactions between drugs and proteins and by CQDS hydrothermal synthesis of nitrogen doped to achieve the measurement of Hg2+. This thesis is divided into four chapters. The first chapter: To summarize the serum albumin, structure of acetylcholinesterase and, the paper outlines the spectral method to explore the interactions between protein and drug content. The mechanism and properties of c-dots, synthesis method and use value in the relevant fields are summarized. The second chapter: the interaction between bovine serum albumin and losartan to explore the spectrum means different, effects of flavonoids rutin the presence of baicalin and its interaction interference. Losartan potassium can obviously cause the fluorescence quenching of bovine serum albumin, proved that the quenching mechanism of bovine serum albumin for losartan potassium A static quenching process. The thermodynamic parameters of H (-134.3kJ mol-1), S (-368.4J.mol-1.K-1) and G (-24.52kJ mol-1~-20.83kJ mol-1), showed that the main force between losartan and bovine serum albumin as van Edward and hydrogen bonding. In addition, bovine serum albumin and potassium chloride candesartan combination between the distance r is 3.183nm that suggests the possibility of energy transfer occurs between them. When in the presence of rutin and baicalin conditions, reduce the binding constant of bovine serum albumin with losartan potassium distance becomes 3.225nm and 3.494nm. addition of bovine serum albumin with losartan potassium, indicating losartan potassium, rutin and baicalin compete in bovine serum albumin above finally, the synchronous fluorescence and three-dimensional spectral to explore the effects of drugs with bovine serum albumin before and after the change of bovine serum albumin structure. The third chapter: by fluorescence spectroscopy, UV Vis absorption spectroscopy and circular two chromatography on baicalin and acetylcholinesterase (AChE) interaction, the mechanism of their action, and the binding constants, binding sites and binding distances were calculated, the baicalin obviously quenches the intrinsic fluorescence quenching of acetylcholinesterase. The main mechanism is static quenching. The thermodynamic parameters of H (-174.6kJ mol-1), S (-489.9 J mol-1.K-1) and G (-28.61kJ mol-1~-23.71kJ mol-1), shows that the force of baicalin and acetylcholinesterase among the van Edward force and hydrogen bond. In addition, acetylcholinesterase and baicalin combined with distance R 2.85nm instructions the energy transfer occurs between them a great chance. By synchronous fluorescence, three-dimensional fluorescence spectroscopy and circular chromatography on two baicalin and AChE activity before and after the changes of acetylcholine esterase. The conformation After the activity of AChE was studied, the IC50 124 M. fourth chapter: by hydrothermal method with serine and ethylenediamine as fluorescent carbon quantum dots synthesized water soluble nitrogen doped well. In quantum yield the optimum excitation wavelength of 360nm under the nitrogen doped carbon quantum dots is 8.66% and by transmission electron microscopy, high resolution transmission electron microscopy, infrared spectroscopy and fluorescence spectroscopy were used to characterize the system, indicates that the carbon quantum dots on the surface of the nitrogen doped rich hydroxyl and carboxyl groups, so the good water solubility. Experiments show that the fluorescence intensity of the mercury ion nitrogen doping carbon quantum dots decreased, the linear range of 1-20 M and a detection limit of 7.33 * 10-5 mol - L-1, it is indicated that nitrogen doped carbon quantum dots has potential applications in ion detection.

【學位授予單位】：河北大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R96;O657.3

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