本文關(guān)鍵詞： 酒精中毒 氨基酸類(lèi) 海馬齒狀回區(qū) 腦部微量透析 二氫麥角堿　出處：《中風(fēng)與神經(jīng)疾病雜志》2015年03期 　論文類(lèi)型：期刊論文

【摘要】：目的觀(guān)察雙氫麥角堿對(duì)急性酒精中毒大鼠海馬齒狀回(dentate gyrus,DG)區(qū)細(xì)胞外液部分氨基酸含量的影響。方法將成年Wistar大鼠(270±30 g)18只隨機(jī)分成對(duì)照組(n=6)、酒精組(n=6)和治療組(n=6)。酒精組與治療組大鼠胃內(nèi)先灌入50%食用酒精,每次0.5 ml,每10 min灌1次,直到大鼠出現(xiàn)酒精中毒狀態(tài)為止。30 min后,治療組大鼠灌胃雙氫麥角堿0.7 mg/kg,酒精組灌胃同等劑量的生理鹽水,對(duì)照組給予同等劑量的生理鹽水。各組動(dòng)物用腦內(nèi)微量透析法及高效液相色譜法測(cè)定海馬DG區(qū)細(xì)胞外液中的幾種氨基酸類(lèi)神經(jīng)遞質(zhì)的含量,包括谷氨酸(glutamate,Glu)、天冬氨酸(aspartate,Asp)、谷氨酰胺(Glutamine,Gln)、甘氨酸(Glycine,Gly)、牛黃酸(Taurine,Tau)和丙氨酸(Alanine,Ala)。結(jié)果 (1)與對(duì)照組相比,急性酒精中毒時(shí)大鼠海馬DG區(qū)細(xì)胞外液中的Asp、Glu和Gly的含量均明顯增加(均為P0.05),而Gln、Tau和Ala含量無(wú)明顯變化(均為P0.05)。(2)治療組大鼠海馬DG區(qū)細(xì)胞外液中的Asp、Glu和Gly的含量與對(duì)照組相比無(wú)顯著差異(均為P0.05)。結(jié)論(1)急性酒精中毒與海馬DG區(qū)Asp、Glu和Gly含量增加有關(guān)。(2)雙氫麥角堿可降低急性酒精中毒導(dǎo)致的大鼠海馬DG區(qū)Asp、Glu和Gly含量。
[Abstract]:Objective to observe the effect of dihydroergol on dentate gyrus in rats with acute alcoholism. Methods Eighteen adult Wistar rats were randomly divided into control group (n = 6). The alcohol group and the treatment group were fed with 50% edible alcohol, once every 10 min each time, 50% rats in the alcohol group and the treatment group were injected into the stomach of the rats of the alcohol group (n = 6) and the treatment group (n = 6). After 30 min of alcohol intoxication, rats in the treatment group were given dihydroergol 0.7 mg / kg, and the alcohol group received the same dose of normal saline. Rats in the control group were given the same dose of normal saline. The contents of several amino acid neurotransmitters in extracellular fluid of hippocampal DG region were determined by microdialysis and high performance liquid chromatography in each group. These include glutamate glutamate Gluten, aspartate Aspen, glutamine glutamate Glutamine glutamate (Gln). Results compared with the control group, glycine Glycine Glycine, Taurine Tauand Alanine Alanine Alaa were compared with the control group. In acute alcoholism, the contents of AspP Glu and Gly in extracellular fluid of rat hippocampal DG region increased significantly (both P0.05A and Gln). There was no significant change in the contents of Tau and Ala (both P0.05An. 2) Asp in extracellular fluid of hippocampal DG region of rats in the treatment group. There was no significant difference in the contents of Glu and Gly between the control group and the control group (P0.050.Conclusion) acute alcoholism and Asp in the DG region of hippocampus. The increase of Glu and Gly contents related to. 2) Dihydroergol can reduce the content of Asp1 Glu and Gly in the DG region of hippocampus induced by acute alcoholism in rats.
【作者單位】： 延邊大學(xué)附屬醫(yī)院神經(jīng)內(nèi)科;
【分類(lèi)號(hào)】：R965
【正文快照】： 急性酒精中毒(Acute alcohol intoxication),是指一次大量飲酒造成的急性中毒,常見(jiàn)于集會(huì)性豪飲過(guò)程中[1]。酒精急性中毒造成中樞神經(jīng)系統(tǒng)全面抑制狀態(tài),并非人們普遍認(rèn)為的中樞興奮性作用[2]。在中樞神經(jīng)系統(tǒng)內(nèi)廣泛存在著氨基酸類(lèi)神經(jīng)遞質(zhì),它們具有代謝作用,還作為神經(jīng)遞質(zhì)對(duì)

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