本文關(guān)鍵詞： 高效液相色譜法 復(fù)方氯霉素滴眼液 氯霉素 醋酸潑尼松龍 氯霉素二醇物 含量測定　出處：《中國藥房》2017年03期 　論文類型：期刊論文

【摘要】：目的:建立同時測定復(fù)方氯霉素滴眼液中氯霉素、醋酸潑尼松龍及其降解產(chǎn)物氯霉素二醇物含量的方法。方法:采用高效液相色譜法。色譜柱為Dionex Acclaim C_(18),流動相為0.1%庚烷磺酸鈉溶液-冰乙酸-乙腈-甲醇(60∶0.2∶30∶10,V/V/V/V),流速為1.0 mL/min,檢測波長為271 nm,柱溫為30℃,進樣量為10μL。結(jié)果:氯霉素、醋酸潑尼松龍、氯霉素二醇物檢測質(zhì)量濃度線性范圍分別為11.73~703.71μg/mL(r=0.999 8)、12.40~744.06μg/mL(r=0.999 8)、1.03~62.03μg/mL(r=0.999 8);氯霉素二醇物的定量限為0.01μg/mL;精密度、穩(wěn)定性試驗的RSD2.0%;氯霉素、醋酸潑尼松龍重復(fù)性試驗的RSD2.0%,氯霉素二醇物的RSD=4.5%;回收率分別為100.6%~102.7%(RSD=0.60%,n=9)、97.5%~100.3%(RSD=0.80%,n=9)、100.0%~103.2%(RSD=1.10%,n=9)。結(jié)論:該方法結(jié)果準確、操作簡便,可用于復(fù)方氯霉素滴眼液中氯霉素、醋酸潑尼松龍及其降解產(chǎn)物氯霉素二醇物含量的同時測定。
[Abstract]:Objective: to establish a simultaneous determination of chloramphenicol in compound chloramphenicol eye drops. Method of determination of chloramphenicol diol in prednisolone acetate and its degradation product. Methods: high performance liquid chromatography was used. The chromatographic column was Dionex Acclaim Che 18). The mobile phase consisted of 0.1% sodium heptane sulfonate solution, glacial acetic acid, acetonitrile-methanol, 60: 0.2: 30: 10 V / V / V / V / V / V / V, the flow rate was 1.0 mL/min. The detection wavelength was 271 nm, the column temperature was 30 鈩，

本文編號：1475685