發(fā)布時間：2018-01-28 01:30

  本文關(guān)鍵詞： 質(zhì)譜成像 空氣動力輔助離子化(AFAI) 藥物分析 代謝產(chǎn)物 內(nèi)源性代謝物　出處：《質(zhì)譜學(xué)報》2017年04期 　論文類型：期刊論文

【摘要】：質(zhì)譜分子成像技術(shù)作為體內(nèi)藥物分析的新興工具受到越來越多的關(guān)注,其中生物體內(nèi)復(fù)雜基質(zhì)的干擾是面臨的關(guān)鍵問題之一。本研究利用高分辨質(zhì)譜技術(shù)的優(yōu)勢,采用空氣動力輔助離子化質(zhì)譜成像方法(AFAI-MSI),建立了高特異性的整體動物體內(nèi)藥物成像分析新方法,以提高體內(nèi)藥物成像分析結(jié)果的準(zhǔn)確性和可靠性。以候選新藥右旋娃兒藤寧堿(S-(+)-deoxytylophorinidine,CAT)為研究對象,采用高分辨質(zhì)譜全掃描與靶向掃描相結(jié)合的方式,對整體動物體內(nèi)藥物的分布進(jìn)行AFAI-MSI分析。結(jié)果表明,該方法能夠準(zhǔn)確地反映藥物特異性分布和處置情況。通過一次質(zhì)譜成像實(shí)驗(yàn),從整體動物水平同時實(shí)現(xiàn)了候選新藥體內(nèi)分布和藥物干預(yù)下內(nèi)源性代謝物的成像分析,為候選新藥的早期研發(fā)提供思路與手段。
[Abstract]:As a new tool of drug analysis in vivo, mass spectrometry molecular imaging technology has attracted more and more attention. The interference of complex matrix in biological body is one of the key problems. In this study, the advantage of high resolution mass spectrometry (HRMS) is used to use aerodynamic assisted ionization mass spectrometry imaging (AFAI-MSI). A new method for the analysis of in vivo drug imaging of whole animal was established. In order to improve the accuracy and reliability of in vivo drug imaging analysis, a new drug, dextroverine, S-deoxytylophorinidine, was used. High resolution mass spectrometry (HRMS) combined with targeted scanning was used to analyze the distribution of drugs in whole animal body by AFAI-MSI. This method can accurately reflect the specific distribution and disposal of drugs. The imaging analysis of endogenous metabolites under drug intervention and distribution in vivo were realized simultaneously from the whole animal level, which provided ideas and means for the early research and development of new drug candidates.
【正文快照】： 　 第 ３８ 卷 第 ４ 期 質(zhì) 譜 學(xué) 報 Ｖｏｌ．３８�。危铮� 　　２０１７ 年 ７ 月 Ｊｏｕｒｎａｌ�。铮妗。茫瑁椋睿澹螅濉。停幔螅蟆。樱穑澹悖簦颍铮恚澹簦颍� Ｓｏｃｉｅｔｙ　 Ｊ ｕｌ．２０１７ 基于高分辨質(zhì)譜技術(shù)的 整體動物體內(nèi)藥物成像分，

本文編號：1469382