發(fā)布時(shí)間：2018-01-23 11:11

  本文關(guān)鍵詞： 番茄皂苷 急性毒性 亞急性毒性 小鼠　出處：《食品科技》2017年11期 　論文類型：期刊論文

【摘要】：目的:研究番茄皂苷對(duì)小鼠急性毒性以及亞急性毒性的影響,為番茄皂苷的安全性作出初步評(píng)價(jià)。方法:急性毒性實(shí)驗(yàn)中,以劑量1.25、2.50、5.00 g/kg對(duì)小鼠一次性灌胃給藥,給藥后連續(xù)觀察7 d,按時(shí)記錄小鼠飲食飲水量和小鼠最終死亡率。觀察結(jié)束后,將小鼠解剖,取心、肝、脾、腎進(jìn)行肉眼觀察,計(jì)算臟器系數(shù),大致確定毒性靶器官。亞急性毒性實(shí)驗(yàn)中,以劑量0.50、2.00、5.00 g/kg對(duì)小鼠灌胃給藥,連續(xù)給藥30 d后,進(jìn)行常規(guī)的血液生化指標(biāo)測(cè)定并計(jì)算主要臟器系數(shù)以及對(duì)肝臟進(jìn)行組織病理學(xué)檢測(cè)。結(jié)果:急性毒性實(shí)驗(yàn)中,3組給藥小鼠未見明顯毒性反應(yīng),飲食飲水量與空白組比較均沒有顯著性差異;整個(gè)實(shí)驗(yàn)過程中,小鼠無1例死亡,番茄皂苷的致死量大于5.00 g/kg;雄性高劑量組小鼠的肝臟指數(shù)與空白組比較明顯增大,有統(tǒng)計(jì)學(xué)意義,其余臟器系數(shù)與空白組對(duì)照未見明顯異常。亞急性毒性實(shí)驗(yàn)中,除了雄性高劑量組小鼠的肝臟系數(shù)與空白組比較存在顯著性差異,其余臟器系數(shù)均無統(tǒng)計(jì)學(xué)意義;血液生化指標(biāo)測(cè)試中,雄、雌性3組給藥組的血尿素氮的含量與空白組比較均降低,有統(tǒng)計(jì)學(xué)意義,其他指標(biāo)未見異常;肝臟病理切片結(jié)果顯示,各給藥組均沒有明顯的肝損傷作用。結(jié)論:番茄皂苷安全無毒,可放心使用。
[Abstract]:Objective: to study the effects of tomato saponins on acute toxicity and subacute toxicity in mice and to evaluate the safety of tomato saponins. The mice were given 5. 00 g / kg intragastric administration for 7 consecutive days. The mice diet and the final mortality were recorded on time. After the observation, the mice were dissected, heart, liver and spleen were taken. Kidney was observed with naked eye, organ coefficient was calculated, and toxic target organ was roughly determined. In subacute toxicity experiment, mice were given intragastric administration with dose of 0.50 ~ 2.00 ~ 5.00 g / kg. After 30 days of continuous administration, routine blood biochemical indexes were measured, the main organ coefficients were calculated and the liver histopathology was detected. Results: in the acute toxicity test. There was no obvious toxic reaction in the three groups, and there was no significant difference between the diet and the blank group. During the whole experiment, there was no death in mice. The lethal dose of tomato saponins was more than 5.00 g / kg. Compared with the blank group, the liver index of the male high-dose group was significantly increased, and the other organ coefficients were not significantly abnormal compared with the blank group. Subacute toxicity test. There was no significant difference in liver coefficient between male high dose group and blank group, but there was no significant difference in other organ coefficients. In the blood biochemical index test, the blood urea nitrogen content in the male and female groups was lower than that in the blank group, and the other indexes were not abnormal. The pathological sections of liver showed that there was no obvious liver injury in each administration group. Conclusion: tomato saponins are safe and nontoxic and can be safely used.
【作者單位】： 廣西植物功能物質(zhì)研究與利用重點(diǎn)實(shí)驗(yàn)室廣西壯族自治區(qū)中國(guó)科學(xué)院廣西植物研究所;廣西中醫(yī)藥大學(xué);
【基金】：廣西科技創(chuàng)新能力與條件建設(shè)計(jì)劃項(xiàng)目(桂科能1598025-32) 廣西壯族自治區(qū)主席科技基金項(xiàng)目(16449-08) 廣西自然科學(xué)基金項(xiàng)目(2015GSNSFBA139120) 廣西植物功能物質(zhì)研究與利用重點(diǎn)實(shí)驗(yàn)室主任基金項(xiàng)目(ZRJJ2014-5,ZRJJ2016-17)
【分類號(hào)】：R99
【正文快照】： 番茄是我們?nèi)粘Ｊ秤米疃嗟囊荒晟卟酥?其果實(shí)營(yíng)養(yǎng)豐富,具有特殊風(fēng)味,可以生食、煮食、加工制成番茄醬、番茄汁等。一般認(rèn)為番茄中主要的功效成分是番茄紅素,其為胡蘿卜素的一種。番茄紅素是一種脂溶性的不飽和碳?xì)浠衔?在番茄中除了脂溶性化合物外尚存在有含量并不低的

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