本文關(guān)鍵詞： 鹽蒿提取液 抑菌活性 抑菌機理　出處：《江蘇農(nóng)業(yè)學(xué)報》2017年04期 　論文類型：期刊論文

【摘要】：以5種常見的致病菌為試驗對象,采用體外抑菌試驗篩選出鹽蒿提取液作用效果最好的致病菌,并通過研究鹽蒿提取液對致病菌作用前后細菌培養(yǎng)液的OD260、電導(dǎo)率、堿性磷酸酶(AKP)活性和細胞超微結(jié)構(gòu)的變化,初步探討鹽蒿提取液對單增李斯特菌(Listeria monocytogenes)的抑菌機制。結(jié)果顯示,鹽蒿提取液對5種致病菌均有抑菌活性,但對單增李斯特菌抑制效果最好,最小抑菌濃度(MIC)為0.062 5 mg/ml,對5種致病菌抑制效果表現(xiàn)為:單增李斯特菌金黃色葡萄球菌大腸桿菌腸炎沙門氏菌鮑氏志賀氏菌。單增李斯特菌經(jīng)鹽蒿提取液處理后,細菌培養(yǎng)液260 nm的吸光度、電導(dǎo)率和AKP活性均增大,表明鹽蒿提取液可破壞單增李斯特菌細胞壁和細胞膜的結(jié)構(gòu),導(dǎo)致細胞通透性增加,進而使細胞內(nèi)容物外泄,使細胞生長受到抑制,進而導(dǎo)致死亡。
[Abstract]:With five common pathogenic bacteria as the experimental object, the bacteria with the best effect of extract of Artemisia Salina were screened by in vitro bacteriostasis test. The changes of OD260, electrical conductivity, alkaline phosphatase (ALP) activity and ultrastructure of bacteria culture medium before and after the action of Artemisia Salina extract on pathogenic bacteria were studied. The antibacterial mechanism of Artemisia Salina extract against Listeria monocytogenes was studied. The results showed that the extracts of Artemisia Salina had antimicrobial activity against five pathogenic bacteria. But the inhibitory effect on Listeria monocytogenes was the best, the minimum inhibitory concentration was 0.062 5 mg/ml. The inhibitive effects on 5 pathogenic bacteria were as follows: Listeria monocytogenes Staphylococcus aureus Escherichia coli Shigella baumannii and Listeria monocytogenes treated with Artemisia Salina extract. The absorbance, conductivity and AKP activity of bacterial culture medium increased at 260nm, which indicated that the extract of Artemisia Salina could destroy the structure of cell wall and cell membrane of Listeria monocytogenes, resulting in the increase of cell permeability. In turn, the cell contents leak out, so that cell growth is inhibited, leading to death.
【作者單位】： 江蘇省農(nóng)業(yè)科學(xué)院農(nóng)產(chǎn)品加工研究所;江蘇海苑食品有限公司;
【基金】：2015年國家留學(xué)基金項目(201508320085) 2014年蘇北科技發(fā)展計劃(富民強縣)項目(BN2014147)
【分類號】：R915
【正文快照】： 鹽蒿(Artemisia halodendron)又名堿蓬、龍須菜,屬一年生草本植物,是一種生長于鹽堿地和海濱沙灘的抗逆性較強的鹽生植物[1]。中國鹽蒿資源豐富,共有鹽蒿屬植物21種和1個變種[2],江蘇擁有中國最大的沿海灘涂濕地,野生鹽蒿的平均產(chǎn)量為1.5 t/hm2,產(chǎn)量十分可觀[3]。鹽蒿含有很多，

本文編號：1450669