本文關(guān)鍵詞：地喹氯銨對(duì)腦膠質(zhì)瘤細(xì)胞凋亡的影響　出處：《中國(guó)老年學(xué)雜志》2017年04期 　論文類型：期刊論文

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【摘要】：目的探討地喹氯銨對(duì)腦膠質(zhì)瘤細(xì)胞增殖、凋亡及侵襲的影響。方法用50、100、200μmol/L的地喹氯銨作用于腦膠質(zhì)瘤細(xì)胞U251、U87、C6,作用時(shí)間分別為12、24、48、72、96 h,MTT檢測(cè)細(xì)胞增殖情況。流式細(xì)胞儀檢測(cè)76μmol/L的地喹氯銨作用48 h后的腦膠質(zhì)瘤細(xì)胞U251的細(xì)胞凋亡情況。Transwell小室檢測(cè)地喹氯銨對(duì)腦膠質(zhì)瘤細(xì)胞侵襲能力的影響。Western印跡檢測(cè)細(xì)胞中PI3K、p-PI3K、Akt、p-Akt蛋白的表達(dá)水平。結(jié)果 50、100、200μmol/L的地喹氯銨對(duì)U251、U87、C6腦膠質(zhì)瘤細(xì)胞增殖均具有抑制作用。地喹氯銨對(duì)腦膠質(zhì)瘤細(xì)胞的增殖抑制作用隨著作用時(shí)間的增加而增加,隨著藥物作用濃度的增加而增加。地喹氯銨作用膠質(zhì)瘤U251、U87、C6細(xì)胞48 h的半數(shù)抑制濃度由大到小依次為:C6U87U251。76μmol/L的地喹氯銨作用后的腦膠質(zhì)瘤細(xì)胞凋亡率明顯高于對(duì)照組(P0.01)。腦膠質(zhì)瘤細(xì)胞經(jīng)地喹氯銨作用后的侵襲細(xì)胞數(shù)明顯低于對(duì)照組(P0.01)。地喹氯銨作用后的腦膠質(zhì)瘤細(xì)胞中PI3K、Akt的表達(dá)水平與對(duì)照組相比無明顯差異,而p-PI3K、p-Akt的表達(dá)水平明顯下降。結(jié)論地喹氯銨對(duì)腦膠質(zhì)瘤細(xì)胞增殖、侵襲能力具有抑制作用,對(duì)腦膠質(zhì)瘤細(xì)胞凋亡具有促進(jìn)作用,作用機(jī)制與PI3K/Akt信號(hào)通路有關(guān)。
[Abstract]:Objective to investigate the effects of diammonium chloride on the proliferation, apoptosis and invasion of glioma cells. Methods 50,100,200 渭 mol/L of diammonium chloride was used to treat the glioma cell line U251 U87. The action time of C _ 6 was 12 ~ (24) C _ (24) O _ (48) C _ (2) H _ (72) ~ (96) h. MTT was used to detect cell proliferation. Flow cytometry was used to detect the action of dilqualkonium chloride at 76 渭 mol/L. Apoptosis of human glioma cell U251 after h .Transwell chamber assay of the effect of diammonium chloride on invasion of glioma cells. Western blot analysis of PI3 in human glioma cells. K. Results the expression level of p-PI3KCX AktPI3KGK and p-Akt protein was 50,100,200 渭 mol/L, and the expression level of p-PI3KT was 50,100,200 渭 mol/L on U251 U87. The proliferation of C6 glioma cells was inhibited, and the inhibitory effect of dilqualkonium chloride on the proliferation of glioma cells increased with the increase of the time of action. With the increase of drug action concentration, the glioma U251 U87 was induced by dilaquinamide. The apoptosis rate of glioma cells was significantly higher than that of the control group (P < 0.05). The half inhibitory concentration of C6 cells was higher than that of the control group (P < 0.05). The apoptosis rate of glioma cells was significantly higher than that of the control group (P < 0.01). The number of invasive cells in glioma cells treated with diqualone was significantly lower than that in the control group (P 0.01). The number of PI3K in glioma cells treated with diquinolium chloride was significantly lower than that in the control group. The expression level of Akt was not significantly different from that of the control group, but the expression level of p-PI3KN p-Akt was significantly decreased. Conclusion Diqualkonium chloride has an inhibitory effect on the proliferation and invasion of glioma cells. It can promote the apoptosis of glioma cells, and the mechanism is related to the PI3K/Akt signaling pathway.
【作者單位】： 錦州醫(yī)科大學(xué)藥學(xué)院;錦州醫(yī)科大學(xué)附屬第一醫(yī)院藥品管理科;
【基金】：遼寧省自然科學(xué)基金(No.2014022044)
【分類號(hào)】：R96
【正文快照】： 目前常用的治療腦膠質(zhì)瘤的方法是手術(shù)治療和放療〔1〕,但由于腦膠質(zhì)瘤具有發(fā)病隱匿、生長(zhǎng)速度快、易轉(zhuǎn)移、易復(fù)發(fā)等特點(diǎn),手術(shù)治療和放療效果仍然不理想。地喹氯銨(DECA)是一種廣譜抗菌藥,對(duì)分枝桿菌、革蘭陰性菌、革蘭陽性菌等具有抑制作用〔2〕,對(duì)口腔潰瘍、扁桃體炎、咽炎、

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 陳振華;任春美;萬仁圣;潘月;伍秋香;袁霜雪;王東旭;黃軍;何百成;孫文娟;;PI3K/Akt信號(hào)與吳茱萸堿抑制人結(jié)腸癌細(xì)胞增殖的關(guān)系研究[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2016年05期

2 王睿君;王育民;劉雁飛;高建梅;包麗薇;戴軍有;;Neuropilins-1和Neuropilins-2在人腦膠質(zhì)瘤中的表達(dá)[J];中國(guó)老年學(xué)雜志;2014年07期

相關(guān)碩士學(xué)位論文 前1條

1 宋彥峰;腫瘤細(xì)胞線粒體靶向遞藥系統(tǒng)的構(gòu)建及其抗腫瘤活性研究[D];第四軍醫(yī)大學(xué);2015年

【共引文獻(xiàn)】

相關(guān)期刊論文 前2條

1 張芳芳;李鑫;郭偉英;;地喹氯銨對(duì)腦膠質(zhì)瘤細(xì)胞凋亡的影響[J];中國(guó)老年學(xué)雜志;2017年04期

2 戴燕燕;陳茂華;;人腦膠質(zhì)瘤GDNF基因啟動(dòng)子Ⅰ區(qū)H3組蛋白乙酰化修飾情況研究[J];中國(guó)現(xiàn)代醫(yī)生;2015年35期

【二級(jí)參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 楊秋s，

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