本文關(guān)鍵詞：異常黑膽質(zhì)成熟劑對(duì)抑郁模型大鼠海馬信號(hào)通路的影響　出處：《新疆醫(yī)科大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 異常黑膽質(zhì)成熟劑 抑郁癥模型 cAMP-PKA信號(hào)通路 ERK信號(hào)通路

【摘要】：目的：觀察異常黑膽質(zhì)成熟劑(ASMq)對(duì)抑郁癥大鼠海馬組織中cAMP-PKA, ERK信號(hào)通路的影響,探討異常黑膽質(zhì)成熟劑抗抑郁作用的信號(hào)通路機(jī)制。方法：采用慢性不可預(yù)見性溫和應(yīng)激(CUMS)誘導(dǎo)建立大鼠抑郁癥模型,并用氟西汀(3.5mg/kg),異常黑膽質(zhì)成熟劑高,中,低劑量(6.0、3.0、1.5g/kg)干預(yù)全程,通過Open-field實(shí)驗(yàn)和糖水消耗實(shí)驗(yàn)來評(píng)估大鼠抑郁活動(dòng)狀態(tài)。觀察大鼠海馬組織中cAMP含量,PKA、p-CREB, p-ERK1/2, ERK1/2的表達(dá)。結(jié)果：應(yīng)激結(jié)束后與正常對(duì)照組相比,抑郁癥模型組大鼠體重增加量降低、糖水消耗量減少(p0.01),水平穿越格數(shù)、豎立次數(shù)均明顯降低(p0.01),處于抑郁狀態(tài)；與抑郁癥模型組相比,陽性對(duì)照組和異常黑膽質(zhì)成熟劑中、高劑量組大鼠體重增加量上升、糖水消耗量升高(p0.01),水平穿越格數(shù)、豎立次數(shù)均明顯增加(p0.01)；與正常對(duì)照組相比,抑郁癥模型組大鼠海馬cAMP含量及PKA、p-CREB表達(dá)降低,差異有顯著性(p0.01,p0.05)。與抑郁癥模型組相比,陽性對(duì)照組和異常黑膽質(zhì)成熟劑中、高劑量組大鼠海馬cAMP含量明顯增加(p0.05), PKA、p-CREB表達(dá)明顯升高(分別為p0.01,p0.05)。各組之間ERK1/2的表達(dá)沒有顯著性差異。與正常對(duì)照組相比,抑郁癥模型組大鼠海馬p-ERK1/2表達(dá)明顯下降,差異有顯著性(p0.01,p0.05)；與抑郁癥模型組相比,陽性對(duì)照組、異常黑膽質(zhì)成熟劑中劑量組海馬組織p-ERK1/2表達(dá)量明顯升高,差異有顯著性(p0.01,p0.05)。結(jié)論：異常黑膽質(zhì)成熟劑可能是通過調(diào)節(jié)cAMP-PKA, ERK信號(hào)通路來發(fā)揮抗抑郁作用。
[Abstract]:Objective: to observe the effect of abnormal black bile maturation (ASMQ) on cAMP-PKA, ERK signal pathway in hippocampus of depression rats. Methods: chronic unpredictable mild stress (CUMS) was used to induce the rat model of depression. It was treated with fluoxetine 3.5 mg 路kg ~ (-1) 路kg ~ (-1), high, medium and low dose of fluoxetine 3.5mg 路kg ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) 路kg ~ (-1)). The activity of depression in rats was evaluated by Open-field test and sugar water consumption test. The content of cAMP in hippocampus was determined by PKAP-CREB2, p-ERK1 / 2. The expression of ERK1/2. Results: compared with the normal control group, the weight gain, sugar water consumption and horizontal traversing number of depression model rats were decreased after stress. The number of erections decreased significantly (P 0.01) and was in a state of depression. Compared with the depression model group, in the positive control group and the abnormal black bilirubin maturation agent, the weight gain, the sugar water consumption and the horizontal crossing number of the rats in the high dose group were increased. The number of erections increased significantly (P 0.01). Compared with the normal control group, the content of cAMP and the expression of PKAP-CREB in the hippocampus of the depression model group were significantly lower than those in the normal control group (P 0.01). Compared with the depression model group, compared with the depression model group, the content of cAMP in hippocampus of the high dose group increased significantly in the positive control group and the abnormal black bile maturation agent, and the content of PKA in the hippocampus of the high dose group was significantly higher than that of the depression model group. The expression of p-CREB was significantly increased (p0.01, p0.05, respectively). There was no significant difference in the expression of ERK1/2 between the three groups, and the expression of p-CREB was significantly higher than that in the normal control group. The expression of p-ERK1 / 2 in hippocampus of depression model group was significantly lower than that of control group. Compared with the depression model group, the expression of p-ERK1 / 2 in hippocampal tissue in the medium dose group of abnormal black bile maturation was significantly higher than that in the depression model group (P 0.01). Conclusion: the abnormal melanoglycoid maturation agent may play an antidepressant role by regulating the cAMP-PKA, ERK signaling pathway.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R96

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