本文關(guān)鍵詞：正交試驗(yàn)優(yōu)選蟲草多糖脂質(zhì)體的制備工藝　出處：《海峽藥學(xué)》2016年11期 　論文類型：期刊論文

  更多相關(guān)文章： 發(fā)酵蟲草菌粉多糖脂質(zhì)體 逆向蒸發(fā)法 正交試驗(yàn) 包封率

【摘要】：目的優(yōu)選蟲草多糖脂質(zhì)體制備的最佳工藝。方法采用逆向蒸發(fā)法制備發(fā)酵蟲草菌粉多糖脂質(zhì)體,以包封率為指標(biāo),以藥脂比、卵膽比與水油比為因素,采用L_9(3~4)正交試驗(yàn)設(shè)計(jì)對制備條件進(jìn)行優(yōu)選,魚精蛋白測定發(fā)酵蟲草菌粉多糖脂質(zhì)體的包封率,透射電鏡及馬爾文激光粒度分析儀測定其形態(tài)和粒徑。結(jié)果逆向蒸發(fā)法制備發(fā)酵蟲草菌粉多糖脂質(zhì)體的最佳工藝為大豆卵磷脂與藥物的比例為20∶1,大豆卵磷脂與膽固醇的比例為2∶1,乙醚與磷酸鹽緩沖液的比例為3∶1。結(jié)論采用最佳工藝制備的發(fā)酵蟲草菌粉多糖脂質(zhì)體包封率較高,形態(tài)和粒徑較均勻,重現(xiàn)性好。
[Abstract]:Objective to select the best technology for preparation of polysaccharide liposomes from Cordyceps sinensis. Methods fermentation polysaccharide liposomes from Cordyceps sinensis were prepared by reverse evaporation. The encapsulation efficiency was taken as the index, the ratio of liposomes to liposomes, the ratio of egg to bile and the ratio of water to oil were used as factors. The preparation conditions were optimized by L _ (9) C _ (3) O _ (4) orthogonal design, and the entrapment efficiency of polysaccharide liposomes from fermented Cordyceps sinensis powder was determined by protamine. Results the optimum technology of preparing polysaccharide liposome from fermented Cordyceps sinensis powder by reverse evaporation was that the ratio of soybean lecithin to drug was 20: 1. The ratio of soybean lecithin to cholesterol was 2: 1, and the ratio of ether to phosphate buffer was 3: 1.Conclusion the entrapment efficiency of polysaccharide liposome from fermented Cordyceps sinensis powder prepared by the best technology is higher. The shape and particle size are uniform and the reproducibility is good.
【作者單位】： 江西南昌市第三醫(yī)院;江西中醫(yī)藥大學(xué);
【分類號】：R943
【正文快照】： 蟲草多糖為中藥冬蟲夏草或發(fā)酵蟲草菌粉的有效成分,具有提高免疫力、抗衰老、抗腫瘤、降血糖等活性〔1-4〕,目前大多數(shù)蟲草多糖制劑在使用過程中體內(nèi)吸收消化快,半衰期短,無法達(dá)到高生物利用度,不能充分發(fā)揮其作用。研究蟲草多糖新劑型或選擇一種更好的載體以提高治療效果并降，

本文編號：1426934