本文關(guān)鍵詞：膦�；繉σ阴Ｄ憠A酯酶的抑制作用及中毒酶重活化特性研究——基于效應(yīng)標(biāo)志物質(zhì)譜定量分析技術(shù)　出處：《高等學(xué)校化學(xué)學(xué)報(bào)》2017年05期 　論文類型：期刊論文

  更多相關(guān)文章： 有機(jī)磷農(nóng)藥 肟類化合物 膦�；� 生物標(biāo)志物 液相色譜-質(zhì)譜聯(lián)用

【摘要】：將對氧磷(Paraoxon)與肟類重活化劑雙復(fù)磷(Obidoxime)反應(yīng)制得膦�；�(DEP-obidoxime);利用基于效應(yīng)標(biāo)志物的質(zhì)譜定量技術(shù)研究了膦酰化肟對乙酰膽堿酯酶(AChE)的抑制作用及中毒酶的重活化特性.結(jié)果表明,膦�；烤哂袠O強(qiáng)的AChE抑制毒性,但因膦�；恐卸久概c原有機(jī)磷毒物中毒酶結(jié)構(gòu)相同,故其膦酰化AChE仍可被經(jīng)典重活化劑[如氯解磷定(Pralidoxime)、雙復(fù)磷(Obidoxime)及酰胺磷定(HI-6)]重活化,根據(jù)EC_(50)表征結(jié)果,這3種重活化劑的重活化效果強(qiáng)弱順序依次為氯解磷定HI-6雙復(fù)磷.
[Abstract]:The phosphonyl oxime (DEP-obidoxime) was synthesized by the reaction of paraoxon and oxime reactivator (Oxime). The inhibitory effect of phosphonyl oxime on acetylcholinesterase (ache) and the reactivation characteristics of toxic enzyme were studied by mass spectrometry quantitative technique based on effect markers. Phosphonyl oxime has very strong AChE inhibition toxicity, but because the phosphonyl oxime poisoning enzyme has the same structure as the original organophosphorus poisoning enzyme, its phosphonate AChE can still be used as the classical reactivator. [For example, Pralidoxime, Obidoxime and HI-6) were reactivated and characterized by ECS 50). The reactivation effect of these three reactivators was in the order of chlorolytic phosphoridated HI-6 double phosphorus.
【作者單位】： 軍事醫(yī)學(xué)科學(xué)院毒物藥物研究所;
【基金】：國家自然科學(xué)基金(批準(zhǔn)號(hào):81202248,81302473)資助~~
【分類號(hào)】：O657.63;R96
【正文快照】： 因有機(jī)氯農(nóng)藥導(dǎo)致的嚴(yán)重污染問題,自20世紀(jì)80年代初我國開始停止其使用[1],而有機(jī)磷農(nóng)藥作為一種高效、廣譜的殺蟲劑開始廣泛應(yīng)用于農(nóng)業(yè)、工業(yè)及醫(yī)藥等領(lǐng)域.有機(jī)磷農(nóng)藥是目前世界上生產(chǎn)量和使用量最多的一類農(nóng)藥,我國有機(jī)磷農(nóng)藥產(chǎn)量占全世界總量的1/3.有機(jī)磷農(nóng)藥多屬于劇毒及，

本文編號(hào)：1421173