發(fā)布時間：2018-01-13 14:28

  本文關(guān)鍵詞：農(nóng)達(dá)對小鼠脾臟淋巴細(xì)胞亞群和氧化應(yīng)激狀態(tài)的影響　出處：《環(huán)境與健康雜志》2017年09期 　論文類型：期刊論文

  更多相關(guān)文章： 除草劑 農(nóng)達(dá) 脾臟 淋巴細(xì)胞 氧化應(yīng)激

【摘要】：目的探討農(nóng)達(dá)(roundup,活性成分為草甘膦)對小鼠脾臟淋巴細(xì)胞亞群和氧化應(yīng)激狀態(tài)的影響。方法將48只成熟SPF級雄性Balb/c小鼠隨機(jī)分為陰性對照(蒸餾水)組和20、200、2 000 mg/kg農(nóng)達(dá)染毒組,每組12只,灌胃染毒0.02ml/g,每日1次,連續(xù)染毒30 d。計算脾臟系數(shù),采用流式細(xì)胞儀分析小鼠脾臟CD3~+、CD4~+、CD8~+T細(xì)胞,CD45r~+B細(xì)胞,CD49b~+NK細(xì)胞比例并計算CD4~+T/CD8~+T比值;測定小鼠脾臟中丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽過氧化物酶(GSH-Px)活力。結(jié)果染毒30 d后,各農(nóng)達(dá)染毒組小鼠脾臟重量呈下降趨勢;與陰性對照組相比,200、2 000mg/kg農(nóng)達(dá)染毒組小鼠脾臟CD3~+、CD4~+、CD8~+T細(xì)胞比例明顯升高,CD45r~+B細(xì)胞比例明顯下降,200 mg/kg農(nóng)達(dá)染毒組CD4~+T/CD8~+T比值下降,差異有統(tǒng)計學(xué)意義(P0.01)。各染毒組小鼠脾臟中MDA含量均增加,GSH-Px活力均降低,差異有統(tǒng)計學(xué)意義(P0.01)。結(jié)論除草劑農(nóng)達(dá)可能通過影響小鼠脾臟淋巴細(xì)胞亞群和氧化應(yīng)激狀態(tài),抑制小鼠免疫功能。
[Abstract]:Objective to study Nongda roundup. Effect of glyphosate on spleen lymphocyte subsets and oxidative stress in mice. Methods 48 mature SPF male Balb/c mice were randomly divided into negative control (distilled water). Group and 20. The rats in the 2000.000 mg/kg Nongda group were treated with 0.02ml / g intragastric administration of 0.02ml / g, once a day for 30 days. The spleen coefficient was calculated. CD4 ~ + CD8 ~ T cells in spleen of mice were detected by flow cytometry (FCM) and CD45rB cells were detected by flow cytometry. The percentage of CD49b ~ NK cells and the ratio of CD4 ~ T / CD8T were calculated. The content of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the spleen of mice were determined. The spleen weight of each Nongda group showed a decreasing trend. Compared with the negative control group, the percentage of CD3 ~ + CD4 ~ + CD8 ~ T cells in the spleen of the mice exposed to Nongda was significantly higher than that of the negative control group. The percentage of CD45rB cells decreased significantly in Nongda group (200 mg/kg). The ratio of CD4 ~ T / CD8 ~ T was decreased in Nongda group. The difference was statistically significant (P 0.01). The content of MDA in the spleen of mice exposed to the toxin increased and the activity of GSH-Px decreased. Conclusion the herbicide Nongda may inhibit the immune function of mice by affecting the spleen lymphocyte subsets and oxidative stress state.
【作者單位】： 濟(jì)南大學(xué)山東省醫(yī)學(xué)科學(xué)院醫(yī)學(xué)與生命科學(xué)學(xué)院;山東省醫(yī)學(xué)科學(xué)院山東省職業(yè)衛(wèi)生與職業(yè)病防治研究院;
【基金】：山東省自然科學(xué)基金(ZR2015YL030)
【分類號】：R99
【正文快照】： 草甘膦(glyphosate)是一種低毒、高效的有機(jī)磷類除草劑,市售草甘膦制劑為農(nóng)達(dá)(41%草甘膦異丙胺鹽水劑),在農(nóng)林業(yè)和畜牧業(yè)中應(yīng)用廣泛。美國環(huán)保署(EPA)將草甘膦列為第三類有毒物質(zhì)(低毒性),但由于其大面積、無節(jié)制、不規(guī)范的使用,導(dǎo)致草甘膦及其代謝產(chǎn)物氨甲基膦酸(AMPA)對水體

【相似文獻(xiàn)】

中國期刊全文數(shù)據(jù)庫 前7條

1 徐書顯,王常有,馬素娥,張鳳云,王忠海,趙娟,左連富;細(xì)菌防護(hù)液對照射小鼠脾臟細(xì)胞生物學(xué)效應(yīng)的影響[J];中國微生態(tài)學(xué)雜志;2000年01期

2 吳桐,李曉玉;小鼠脾臟淋巴細(xì)胞上存在μ阿片受體的證據(jù)(英文)[J];Acta Pharmacologica Sinica;1999年09期

3 曾祥元,姚學(xué)文;多抗甲素對小鼠脾臟超微結(jié)構(gòu)的影響[J];抗生素;1983年06期

4 許金花,高永,李冰,趙擁軍;鄰甲苯胺對小鼠脾臟作用的實(shí)驗(yàn)研究[J];濱州醫(yī)學(xué)院學(xué)報;1998年05期

5 賴業(yè)馥;郭禱年;周克倫;;~(60)Co-γ線照射對小鼠脾臟和肝臟腺苷酸環(huán)化酶活性的影響[J];軍事醫(yī)學(xué)科學(xué)院院刊;1980年02期

6 韓艷芳;彭立人;;肉堿對氧化應(yīng)激狀態(tài)的影響[J];國際移植與血液凈化雜志;2006年06期

7 張道權(quán);吳正祥;吳強(qiáng);楊楓;吳正升;;硫酸亞鐵對潰瘍性結(jié)腸炎模型大鼠氧化應(yīng)激狀態(tài)的影響[J];現(xiàn)代醫(yī)學(xué);2007年04期

，

本文編號：1419243