本文關(guān)鍵詞：親和毛細管整體柱在牛血清白蛋白與奈福泮對映體相互作用研究中的應(yīng)用　出處：《中國藥科大學(xué)學(xué)報》2016年01期 　論文類型：期刊論文

  更多相關(guān)文章： 親和毛細管整體柱 手性藥物 牛血清白蛋白 相互作用 奈福泮 前沿分析法

【摘要】：利用胃蛋白酶鍵合的有機聚合物整體柱在電色譜中對手性藥物奈福泮的拆分能力,采用前沿分析法同時對奈福泮的兩個對映體與牛血清白蛋白(BSA)的相互作用情況進行了考察。經(jīng)過優(yōu)化后建立的電色譜條件為:胃蛋白酶修飾的聚(甲基丙烯酸環(huán)氧丙酯-乙二醇二甲基丙烯酸酯)毛細管整體柱作為分離通道(32 cm×75μm,有效長度22 cm),運行緩沖液為pH 5.5的15 mmol/L醋酸銨,樣品溶劑為pH 7.4的50 mmol/L醋酸銨,運行電壓為5.0 kV,電壓進樣10 kV×6 s,檢測波長為215 nm。此時奈福泮兩個對映體平臺峰彼此完全分離,結(jié)合體系中BSA對對映體在電色譜中的分離和檢測均無影響,測得兩個對映體與BSA的結(jié)合常數(shù)分別為443和527 L/mol,結(jié)合位點數(shù)均為1.0,結(jié)合位點為Sudlow siteⅡ。
[Abstract]:The separation ability of using organic polymer monolithic column bonded in pepsin electrochromatography chiral drugs of nefopam, by frontal analysis of nefopam two enantiomers with bovine serum albumin (BSA) interactions were investigated. After power optimized chromatographic conditions established: pepsin modification poly (methacrylic acid ethylene glycol two glycidyl methacrylate) monolithic capillary column as the separation channel (32 cm * 75 m, the effective length of 22 cm), the running buffer was pH 5.5 15 mmol/L ammonium acetate, the sample solvent for pH 7.4, 50 mmol/L ammonium acetate, the operating voltage is 5 kV, voltage. 10 kV * 6 s, the detection wavelength was 215 nm. when nefopam two enantiomers were separated completely from each other with the peak platform, in the system of BSA had no effect on the separation and determination of enantiomers in electro chromatography, measured two enantiomers and combined with BSA The constants are 443 and 527 L/mol respectively, and the number of binding sites is 1, and the binding site is Sudlow site II.

【作者單位】： 中國藥科大學(xué)分析化學(xué)教研室;
【分類號】：R917
【正文快照】： 引用本文曾s，

本文編號：1367610