本文關(guān)鍵詞：地塞米松致大鼠肝臟損傷中肝臟miRNA變化的研究　出處：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 地塞米松 肝損傷 miRNA 測序分析 poly(A)RT-qPCR

【摘要】：目的：本文旨在研究地塞米松導(dǎo)致的大鼠肝臟損傷中肝組織miRNA表達(dá)量的變化。方法：將Wistar大鼠隨機分為兩組：空白對照組(CON組,n=8),皮下注射生理鹽水0.2 ml；地塞米松誘導(dǎo)肝損傷模型組(DEX組,n=8),皮下注射地塞米松磷酸鈉注射液,2.4 mg/kg。連續(xù)注射7天后,禁食不禁水24h,處死大鼠,測定血清丙氨酸轉(zhuǎn)氨酶(ALT)、天冬氨酸轉(zhuǎn)氨酶(AST)、血糖(GLU)及甘油三酯(TG)水平,測定肝臟組織超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量和谷胱甘肽(GSH)含量。通過HE染色觀察肝臟病理學(xué)變化。結(jié)果：(1)DEX組大鼠血清的ALT、AST、TG水平均顯著增高,GLU水平顯著降低；(2)組織切片顯示,與對照組(CON)大鼠相比,注射地塞米松后大鼠肝臟細(xì)胞發(fā)生了變性；(3)通過miRNA測序分析發(fā)現(xiàn),rno-miR-21-5p、rno-miR-215、 rno-miR-29b-5p、rno-miR-499-5p、rno-miR-34a-5p、rno-miR-203b-5p、 rno-miR-100-3p、rno-miR-676、rno-miR-378a-3p和一個未知的miRNA表達(dá)量顯著增高,rno-miR-672-3p、rno-miR-146a-3p、rno-miR-122-5p、rno-miR-205、 mo-let-7b-5p 和 rno-miR-146b-3p表達(dá)量顯著降低；poly(A) RT-qPCR檢測驗證了rno-miR-205、rno-let-7b-5p、rno-miR-672-3p表達(dá)量降低和rno-miR-29b-5p、 rno-miR-34a-5p、rno-miR-676表達(dá)量增高的趨勢；(4)通過對差異表達(dá)miRNA進(jìn)行靶基因預(yù)測和功能注釋,推測到差異表達(dá)miRNA相關(guān)的生物學(xué)功能。結(jié)論：注射地塞米松造成了大鼠肝臟細(xì)胞發(fā)生變性,大鼠肝組織miRNA表達(dá)發(fā)生顯著變化,差異表達(dá)的miRNA與不同的生物學(xué)功能相關(guān)。
[Abstract]:Objective: the aim of this study was to investigate the changes in the expression of miRNA in the liver tissue of rats induced by dexamethasone. Methods: Wistar rats were randomly divided into two groups: blank control group (group CON, n=8), subcutaneous injection of normal saline 0.2 ml, dexamethasone induced liver injury model group (DEX group, n=8), subcutaneous injection of Dexamethasone Sodium Phosphate Injection, 2.4 mg/kg. After 7 days of continuous injection, fasting 24h, rats were killed, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), blood glucose (GLU) and triglyceride (TG) level, the determination of liver tissue superoxide dismutase (SOD) activity, malondialdehyde (MDA) content and glutathione (GSH) content. The pathological changes of liver were observed by HE staining. Results: (1) serum DEX group rats ALT, AST and TG levels were significantly increased, GLU levels decreased significantly; (2) tissue sections showed that, compared with the control group (CON) compared with rats after injection of dexamethasone in rats liver cell degeneration; (3) by miRNA sequencing analysis in this paper. Rno-miR-21-5p, rno-miR-215, rno-miR-29b-5p, rno-miR-499-5p, rno-miR-34a-5p, rno-miR-203b-5p, rno-miR-100-3p, rno-miR-676, rno-miR-378a-3p and an unknown miRNA expression was significantly increased, the expression of rno-miR-672-3p, rno-miR-146a-3p, rno-miR-122-5p, rno-miR-205, mo-let-7b-5p and rno-miR-146b-3p were significantly reduced; poly (A) RT-qPCR, rno-let-7b-5p, rno-miR-205 detection verified the expression of rno-miR-672-3p decreased and rno-miR-29b-5p, rno-miR-34a-5p and the expression of rno-miR-676 increased; (4) the difference in expression of miRNA Target gene prediction and functional annotation were used to speculate on the biological functions of differential expression of miRNA. Conclusion: the injection of dexamethasone induces the degeneration of rat liver cells, and the expression of miRNA is significantly changed in rat liver. The differential expression of miRNA is related to different biological functions.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R965

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