本文選題：間歇低氧　切入點：海馬　出處：《中國神經(jīng)精神疾病雜志》2014年09期 　論文類型：期刊論文

【摘要】：目的探討間歇低氧對大鼠海馬細(xì)胞外信號調(diào)節(jié)激酶(extracellular signal-regulated kinase,ERK)通路及認(rèn)知功能變化的影響。方法采用大鼠間歇低氧模型,將成年雄性Wistar大鼠(n=48)隨機均分為5%間歇低氧組(5%intermittent hypoxia group,5%IH組)和空白對照組(unhandled control group,UC組),每組分別為24只大鼠,分別于間歇低氧第2、4、6、8周后采用Morris水迷宮實驗檢測大鼠學(xué)習(xí)記憶功能;免疫組化及免疫印跡法檢測大鼠海馬區(qū)磷酸化P-ERK1/2表達水平、免疫組化檢測c-fos蛋白表達水平;TUNEL法檢測神經(jīng)元凋亡情況。結(jié)果與UC組比較,5%IH組在2、4、6、8周大鼠逃避潛伏期時間分別為(50.22±8.42)s、(57.13±9.06)s、(67.56±5.42)s、(71.71±5.49)s,從第2周起隨低氧時間延長大鼠逃避潛伏期時間延長(P0.05),5%IH組在2、4、6、8周大鼠跨越目標(biāo)象限時間分別為(46.23±3.68)s、(39.36±3.42)s、(33.35±4.01)s、(26.82±4.30)s,從第2周起隨低氧時間延長大鼠跨越目標(biāo)象限時間縮短(P0.05),5%IH組p-ERKl/2、c-fos蛋白表達及凋亡指數(shù)在2、4、6、8周均多于UC組(P0.05),5%IH組p-ERKl/2、c-fos蛋白表達及凋亡指數(shù)均有明顯的時間差異性(P0.05),均隨間歇低氧時間的延長其表達先升高后降低,6周達到高峰(P0.05),8周逐漸下降(P0.05);UC組p-ERKl/2、c-fos蛋白表達及凋亡指數(shù)無時間差異性(P0.05),5%IH組p-ERKl/2與c-fos蛋白的陽性表達相對值呈正相關(guān)(r值為0.977,P0.05),c-fos蛋白的陽性表達相對值與神經(jīng)元凋亡指數(shù)呈正相關(guān)(r值為0.963,P0.05)。結(jié)論ERK通路的激活可能是OSAHS大鼠早中期認(rèn)知功能損害發(fā)生的重要機制。
[Abstract]:Objective to investigate the effects of intermittent hypoxia on extracellular signal-regulated kinases (ERK) pathway and cognitive function in rat hippocampus. Adult male Wistar rats were randomly divided into 5% intermittent hypoxia group (n = 5) and a blank control group (n = 24). The rats in each group were randomly divided into two groups: the control group (n = 24) and the control group (n = 24). Morris water labyrinth test was used to detect the learning and memory function of the rats after 68 weeks of intermittent hypoxia. The expression of phosphorylated P-ERK1 / 2 in hippocampus of rats was detected by immunohistochemistry and Western blotting. Results compared with UC group, the escape incubation period of rats in 5IH group was 50.22 鹵8.42% and 67.56 鹵5.42 sago respectively, and the escape latency was prolonged with hypoxia time from the 2nd week to the second week. The time of crossing the target quadrant was 46.23 鹵3.68 ~ 39.36 鹵3.42 ~ 39.36 鹵3.42 in the P0.05 / 5IH group, respectively. The expression of p-ERKl-c-fos protein and the apoptosis index in the p-ERKl-2c-fos group were more than those in the P0.05IH group at 2468 weeks with the prolongation of the time of crossing the target quadrant from the second week. The expression of p-ERKl- c-fos protein and the apoptosis index in the P0.05IH group were higher than those in the UC group P0.055IH group with the prolongation of the time of crossing the target quadrant from the 2nd week to the end of the second week. The expression of p-ERKl- 2c-fos protein and the apoptosis index of the rats in the P0.05IH group were higher than those in the UC group at 2468 weeks. White expression and apoptosis index were significantly different in time. With the prolongation of intermittent hypoxic time, the expression of white protein increased first, then decreased to the peak at 6 weeks. The expression of p-ERKl / 2nc-fos protein and apoptosis index in UC group decreased gradually after 8 weeks. There was no significant difference in P0.055and IH. The positive correlation between the expression of p-ERKl / 2 and c-fos protein was positively correlated with the expression of c-fos protein. The positive correlation between the expression of p-ERKl / 2 and the expression of c-fos protein was 0.977P0.05and the neuron apoptosis index. Conclusion the activation of ERK pathway may be the cognitive impairment of OSAHS rats in early and middle stage. An important mechanism for the occurrence of damage.
【作者單位】： 河北聯(lián)合大學(xué)附屬醫(yī)院呼吸科;
【基金】：河北省高等學(xué)校科學(xué)技術(shù)研究重點項目 河北省重大課題資助項目 河北省自然科學(xué)基金資助項目(編號:ZH2011120;zd2013091;H2014209231)
【分類號】：R766

【共引文獻】

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本文編號：1621371