發(fā)布時間：2024-03-07 19:41

　　心肌梗死是當今社會中引發(fā)死亡的重要因素，因此開發(fā)新的治療方法是臨床和研究心肌梗死的重要目標。心肌梗死涉及到的相關(guān)基因和過程眾多，其中的調(diào)控關(guān)系也很復雜。本文中，我們基于基因調(diào)控網(wǎng)絡(luò)的方法對心肌梗死中的基因調(diào)控模式進行了綜合分析。首先，我們從多個資源如：PubMed、CADgene、GWAS、HMDD and miR2disease收集了與心肌梗死相關(guān)的基因和miRNA。其次，我們預測了人類所有轉(zhuǎn)錄因子、心肌梗死相關(guān)的miRNA及基因間的調(diào)控關(guān)系。轉(zhuǎn)錄因子對心肌梗死相關(guān)的miRNA和基因的調(diào)控，我們從UCSC（hg18）獲得了預測的轉(zhuǎn)錄因子結(jié)合位點以及從TRANSFAC和ChIP-seq數(shù)據(jù)獲得了轉(zhuǎn)錄因子驗證的靶基因。通過整合兩個miRNA靶基因預測軟件TargetScan和miRanda的結(jié)果，得到了miRNA預測的靶基因，并整合miR2Disease、miRTarBase、miRecords和TarBase四個數(shù)據(jù)庫收集的實驗驗證的miRNA靶基因，我們獲得了心肌梗死相關(guān)miRNA的靶基因。最后，我們使用上述調(diào)控關(guān)系構(gòu)建了心肌梗死特異的miRNA-轉(zhuǎn)錄因子共調(diào)控網(wǎng)絡(luò)。該網(wǎng)絡(luò)共包含了2...

【文章頁數(shù)】：68 頁

【學位級別】：碩士

【文章目錄】：
Abstract
摘要
Contents
CHAPTER 1: INTRODUCTION AND LITERATURE REVIEW
    1.1 INTRODUCTION
    1.2 MicroRNAs and Transcription factors in cardiovascular diseases
    1.3 Myocardial Infarction
    1.4 Ventricular Remodeling
    1.5 Current cardioprotective agents and therapeutic strategies essential in myocardial ischemia/reperfusion (I/R) injury
    1.6 Construction method of microRNA and Transcription factor co‐regulatory networks
    1.7 Study Objectives
CHAPTER 2: METHODOLOGY
    2.1 MI candidate genes and miRNAs
    2.2 Identification of miRNA and TF targets
    2.3 Network and hub analysis
    2.4 Functional Annotation of miRNA targets and pathway analysis
CHAPTER 3: RESULTS
    3.1 Basic information of MI related miRNAs and genes curated from literature and databases ..
    3.2 MI miRNA‐TF regulatory network and its characteristics
    3.3 Overlap between hub genes in the MI miRNA‐TF regulatory network
    3.4 Post‐MI subnetwork and overlap between MI hub and Post‐MI hub subnetworks
    3.5 Canonical pathway enrichment of miRNA and TF targets
    3.6 NFAT and Hypertrophy of the heart specific regulatory network in MI
    3.7 Cellular Model of essential components in MI progression
CHAPTER 4: DISCUSSION AND CONCLUSION
Acknowledgements
References



本文編號：3921644