天堂国产午夜亚洲专区-少妇人妻综合久久蜜臀-国产成人户外露出视频在线-国产91传媒一区二区三区

miR-24對(duì)人臍靜脈內(nèi)皮細(xì)胞增殖、轉(zhuǎn)移及自噬的影響

發(fā)布時(shí)間:2019-03-13 09:08
【摘要】:目的探討miR-24對(duì)人臍靜脈內(nèi)皮細(xì)胞(HUVECs)增殖、轉(zhuǎn)移、自噬的影響。方法將HUVECs隨機(jī)分為空白對(duì)照組、雷帕霉素+miR-24高表達(dá)組、雷帕霉素組。空白對(duì)照組不給予任何處理;雷帕霉素組的HUVECs用1 000 nmol/L雷帕霉素處理6 h建立自噬模型。雷帕霉素+miR-24高表達(dá)組的HUVECs先轉(zhuǎn)染miR-24高表達(dá)質(zhì)粒,待轉(zhuǎn)染成功后以同樣方法建立自噬模型;用CCK-8法檢測(cè)HUVECs增殖能力,細(xì)胞劃痕試驗(yàn)檢驗(yàn)細(xì)胞的轉(zhuǎn)移能力,進(jìn)一步用免疫組織化學(xué)和Western blotting法檢測(cè)HUVECs自噬蛋白微管相關(guān)蛋白1輕鏈3(LC3)和Beclin-1的蛋白表達(dá)水平,采用透射電鏡觀察細(xì)胞內(nèi)部自噬小體。結(jié)果與空白對(duì)照組相比,雷帕霉素組、雷帕霉素+miR-24高表達(dá)組OD值、細(xì)胞遷移比例降低、Beclin-1蛋白及LC3Ⅱ蛋白表達(dá)量上升(P0.01或0.05)。與雷帕霉素組相比,雷帕霉素+miR-24高表達(dá)組OD值、細(xì)胞遷移比例下降,Beclin-1、LC3Ⅱ蛋白表達(dá)量下降(P0.01或0.05)。透射電鏡觀察結(jié)果顯示,空白對(duì)照組和雷帕霉素+miR-24高表達(dá)組的細(xì)胞質(zhì)內(nèi)各細(xì)胞器分布基本正常,細(xì)胞核形態(tài)也正常,細(xì)胞質(zhì)中未見(jiàn)明顯自噬小體;雷帕霉素組細(xì)胞質(zhì)中可見(jiàn)明顯的空泡狀結(jié)構(gòu)、包含部分雙層膜結(jié)構(gòu)的自噬小體。結(jié)論 miR-24可顯著抑制HUVECs的增殖、轉(zhuǎn)移及自噬。
[Abstract]:Objective to investigate the effects of miR-24 on proliferation, metastasis and autophagy of human umbilical vein endothelial cells (HUVECs). Methods HUVECs was randomly divided into blank control group, rapamycin miR-24 overexpression group and rapamycin group. The HUVECs of rapamycin group was treated with 1 000 nmol/L rapamycin for 6 h to establish autophagy model. The HUVECs of rapamycin miR-24 high expression group was first transfected into miR-24 high expression plasmid, and the autophagy model was established by the same method after the transfection was successful. The proliferation ability of HUVECs was detected by CCK-8 method, the ability of cell metastasis was tested by cell scratch test, and the expression of HUVECs autophagy protein microtubule-associated protein 1 light chain 3 (LC3) and Beclin-1 was detected by immunohistochemistry and Western blotting method. The intracellular autophagy was observed by transmission electron microscopy (TEM). Results compared with the blank control group, the OD value, cell migration ratio and the expression of Beclin-1 protein and LC3鈪,

本文編號(hào):2439257

資料下載
論文發(fā)表

本文鏈接:http://sikaile.net/yixuelunwen/xxg/2439257.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶e868f***提供,本站僅收錄摘要或目錄,作者需要?jiǎng)h除請(qǐng)E-mail郵箱bigeng88@qq.com
欧美特色特黄一级大黄片| 久久热在线视频免费观看| 久久人妻人人澡人人妻| 一区二区三区日本高清| 国产午夜精品亚洲精品国产| 91后入中出内射在线| 午夜精品一区二区三区国产| 亚洲欧美日韩国产自拍| 欧美一区二区三区不卡高清视| 五月的丁香婷婷综合网| 日韩在线欧美一区二区| 91亚洲精品综合久久| 亚洲精品国产美女久久久99| 日本深夜福利视频在线| 99久久国产亚洲综合精品| 男女一进一出午夜视频| 丰满少妇被粗大猛烈进出视频| 暴力性生活在线免费视频| 熟妇人妻av中文字幕老熟妇| 国产精品午夜福利免费在线| 国产偷拍盗摄一区二区| 男人把女人操得嗷嗷叫| 久久精品中文扫妇内射| 欧美自拍偷自拍亚洲精品| 精品久久av一二三区| 69精品一区二区蜜桃视频| 成人三级视频在线观看不卡| 国产亚洲欧美自拍中文自拍| 欧洲一区二区三区蜜桃| 老熟妇乱视频一区二区| 国产精品日韩精品最新| 91后入中出内射在线| 欧美精品在线观看国产| 国产精品一区二区三区日韩av| 亚洲美女国产精品久久| 极品少妇一区二区三区精品视频 | 日韩欧美精品一区二区三区| 国产精品视频第一第二区| 欧美中文字幕日韩精品| 日本免费一区二区三女| 福利一区二区视频在线|