【摘要】：目的:探討壓力超負(fù)荷大鼠心肌膠原代謝的動(dòng)態(tài)變化。方法:采用腹主動(dòng)脈縮窄法制備壓力超負(fù)荷大鼠模型,并以假手術(shù)組為對照,分別于術(shù)后第3周、4周、8周和12周取材,稱量大鼠體質(zhì)量、心臟質(zhì)量和左心室質(zhì)量,計(jì)算心臟質(zhì)量指數(shù)(heart mass index,HMI)和左心室質(zhì)量指數(shù)(left ventricle mass index,LVMI);心肌組織Masson染色觀察心肌膠原纖維沉積情況;樣本堿水解法檢測心肌組織羥脯氨酸(hydroxyproline,HYP)含量;ELISA法檢測血清Ⅰ型前膠原羧基端肽(procollagen typeⅠcarboxy-terminal peptide,PⅠCP)、Ⅲ型前膠原氨基端肽(procollagen typeⅢamino-terminal peptide,PⅢNT)和Ⅰ型膠原C端肽(collagen C telopeptide typeⅠ,CTX-Ⅰ)水平。結(jié)果:與假手術(shù)組相比,模型組大鼠心肌血管周圍及間質(zhì)可見膠原纖維沉積,心肌膠原容積分?jǐn)?shù)明顯增高(P0.01),且隨時(shí)間延長膠原沉積明顯增多;模型組大鼠HMI、LVMI和HYP均明顯增加(P0.05),且隨時(shí)間延長大鼠HYP有升高的趨勢;模型組大鼠血清PⅠCP濃度顯著升高,在第4、8和12周時(shí)差異有統(tǒng)計(jì)學(xué)意義(P0.05);模型組大鼠血清PⅢNP濃度顯著升高,CTX-Ⅰ濃度顯著降低,差異均有統(tǒng)計(jì)學(xué)意義(P0.01)。結(jié)論:壓力超負(fù)荷狀態(tài)下,心肌膠原代謝紊亂,且隨時(shí)間的延長心肌呈不同程度的纖維化表現(xiàn)。
[Abstract]:Objective: to investigate the dynamic changes of myocardial collagen metabolism in pressure overload rats. Methods: the pressure overload rat model was established by abdominal aortic coarctation, and the sham-operated group was used as control group. The rats were measured at the 3rd, 4th, 8th and 12th week after operation to weigh the body mass, heart mass and left ventricular mass. Cardiac mass index (heart mass index,HMI) and left ventricular mass index (left ventricle mass index,LVMI) were calculated. Myocardial collagen deposition was observed by Masson staining and hydroxyproline (hydroxyproline,HYP) content was detected by alkaline hydrolysis. Serum (procollagen type 鈪，

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