乙醛脫氫酶2活性水平與冠心病的相關(guān)性研究
發(fā)布時間：2018-12-05 22:29
【摘要】：目的:采用雙抗體一步夾心法酶聯(lián)免疫吸附試驗,檢測急性心肌梗死組、心絞痛組和正常對照組血漿ALDH2表達水平,分析ALDH2與冠心病各組之間關(guān)系及與冠脈病變嚴重程度的相關(guān)性。方法:選擇2015年3月至2016年10月,因胸痛、胸悶以及冠狀動脈疾病其他癥狀發(fā)病的患者,經(jīng)我院心內(nèi)科醫(yī)師收治于心血管內(nèi)科,所入選的實驗對象共217例,其中男性158例,女性82例,正常對照組共31例,包括男性19例,女性12例,平均年齡64.41±9.0歲,其中心絞痛組共82人,包括男性54例,女性28例,平均年齡63.84±9.24,急性心肌梗死組共104人,包括男性64例,女性40例,平均年齡64.72±9.62。依據(jù)冠脈病變支數(shù)及計算所得Gensini積分總和,分為若干個亞組,各亞組之間用冠脈病變支數(shù)和Gensini積分以評價冠脈病變嚴重程度,即根據(jù)Gensini積分分為冠脈病變輕度組,中度組,重度組,冠脈病變支數(shù)分為:單支病變、雙支病變、三支病變。采用雙抗體一步夾心法酶聯(lián)免疫吸附實驗(ELISA),用酶標儀在450nm波長測定吸光度(OD)值,計算樣品ALDH2水平。結(jié)果:1急性心肌梗死組、心絞痛組、正常對照組檢測ALDH2結(jié)果分別為3.44±1.53、4.08±1.85、7.32±0.34 U/L,其表達活性在AMI組最低,在正常對照組中最高,心絞痛組次之。且AMI組與正常對照組之間存在統(tǒng)計學差異,其P值0.05;心絞痛組與對照組同樣存在統(tǒng)計學意義,P值0.05;但AMI與心絞痛組無統(tǒng)計學意義。2.ALDH2與冠脈病變支數(shù)比較結(jié)果顯示:三支病變ALDH2水平高于其余兩組,但冠脈病變?nèi)M之間比較,無統(tǒng)計學意義。3.ALDH2與冠脈病變嚴重程度比較結(jié)果顯示:病變程度重的患者ALDH2水平最低,中度居中,輕度病變者其活性最高。4.ALDH2多重線性回歸分析結(jié)果顯示:冠心病高血壓和HDL對ALDH2表達水平有影響,差異有統(tǒng)計學意義。5.冠心病包括AMI、心絞痛組與實驗對象的各生化指標指標間單因素分析顯示:HDL水平、ALDH2水平與患者冠心病的發(fā)病有相關(guān)性,P0.05,與其他一般指標無明顯統(tǒng)計學意義。結(jié)論:1.ALDH2在心絞痛組、急性心肌梗死組的表達水平較正常對照組中明顯降低,提示ALDH2在冠心病發(fā)病機制中起重要作用。2.ALDH2水平與冠脈病變嚴重程度呈負相關(guān),有可能成為評估冠心病患者病變嚴重程度的的可檢測指標。3.ALDH2水平與冠脈病變支數(shù)有關(guān)。4.HDL、ALDH2水平心絞痛組、急性心肌梗死組的表達水平較正常對照組中明顯降低,提示HDL可能與ALDH2協(xié)同在冠心病發(fā)病機制中起重要作用。
[Abstract]:Objective: to detect the expression of plasma ALDH2 in acute myocardial infarction (AMI) group, angina pectoris group and normal control group by double antibody one step sandwich enzyme-linked immunosorbent assay (Elisa). To analyze the relationship between ALDH2 and coronary heart disease (CHD) and the relationship between ALDH2 and severity of coronary artery disease. Methods: from March 2015 to October 2016, 217 patients with chest pain, chest tightness and other symptoms of coronary artery disease were admitted to the Department of Cardiovascular Medicine, including 158 males. There were 82 females and 31 normal controls, including 19 males and 12 females, with an average age of 64.41 鹵9.0 years. There were 82 patients with angina pectoris, including 54 males and 28 females, with an average age of 63.84 鹵9.24 years. There were 104 patients in acute myocardial infarction group, including 64 males and 40 females with an average age of 64.72 鹵9.62. According to the number of coronary lesion branches and the sum of calculated Gensini scores, they were divided into several subgroups. The severity of coronary lesions was evaluated by the number of coronary lesion branches and the Gensini score among the subgroups, that is, according to the Gensini score, they were divided into mild coronary lesion group and moderate coronary lesion group. In severe group, coronary artery lesion was divided into single vessel lesion, double vessel lesion and three vessel lesion. Double antibody one step sandwich enzyme-linked immunosorbent assay (ELISA),) was used to determine the absorbance (OD) value at 450nm wavelength and to calculate the ALDH2 level of the sample. Results: 1the results of ALDH2 in acute myocardial infarction group, angina pectoris group and normal control group were 3.44 鹵1.53 鹵4.08 鹵1.85 鹵7.32 鹵0.34 U / L, respectively. The expression of ALDH2 was the lowest in AMI group, the highest in normal control group, followed by angina pectoris group. There was statistical difference between the AMI group and the normal control group (P value 0.05), and the angina pectoris group and the control group also had statistical significance (P value 0.05). However, there was no significant difference between AMI and angina pectoris. The results of 2.ALDH2 and coronary artery disease showed that the level of ALDH2 in three vessel lesions was higher than that in the other two groups, but there was a significant difference among the three groups. There was no significant difference between 3.ALDH2 and severity of coronary artery disease. The results showed that the ALDH2 level of the patients with severe lesions was the lowest, moderate, moderate. The results of 4.ALDH2 multiple linear regression analysis showed that hypertension and HDL of coronary heart disease had influence on the expression of ALDH2, and the difference was statistically significant. Univariate analysis showed that the levels of HDL and ALDH2 were correlated with the incidence of coronary heart disease in patients with coronary heart disease including AMI, angina pectoris group and experimental subjects (P 0.05), but had no statistical significance with other general indexes. Conclusion: the expression of 1.ALDH2 in angina pectoris group and acute myocardial infarction group is significantly lower than that in normal control group, suggesting that ALDH2 plays an important role in the pathogenesis of coronary heart disease. The level of 2.ALDH2 is negatively correlated with the severity of coronary artery disease. The level of 3.ALDH2 is related to the number of coronary artery disease branches. 4. The expression level of 3.ALDH2 in angina pectoris group and acute myocardial infarction group is significantly lower than that in normal control group. The results suggest that HDL and ALDH2 may play an important role in the pathogenesis of coronary heart disease.
【學位授予單位】：蚌埠醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R541.4
本文編號：2365546
[Abstract]:Objective: to detect the expression of plasma ALDH2 in acute myocardial infarction (AMI) group, angina pectoris group and normal control group by double antibody one step sandwich enzyme-linked immunosorbent assay (Elisa). To analyze the relationship between ALDH2 and coronary heart disease (CHD) and the relationship between ALDH2 and severity of coronary artery disease. Methods: from March 2015 to October 2016, 217 patients with chest pain, chest tightness and other symptoms of coronary artery disease were admitted to the Department of Cardiovascular Medicine, including 158 males. There were 82 females and 31 normal controls, including 19 males and 12 females, with an average age of 64.41 鹵9.0 years. There were 82 patients with angina pectoris, including 54 males and 28 females, with an average age of 63.84 鹵9.24 years. There were 104 patients in acute myocardial infarction group, including 64 males and 40 females with an average age of 64.72 鹵9.62. According to the number of coronary lesion branches and the sum of calculated Gensini scores, they were divided into several subgroups. The severity of coronary lesions was evaluated by the number of coronary lesion branches and the Gensini score among the subgroups, that is, according to the Gensini score, they were divided into mild coronary lesion group and moderate coronary lesion group. In severe group, coronary artery lesion was divided into single vessel lesion, double vessel lesion and three vessel lesion. Double antibody one step sandwich enzyme-linked immunosorbent assay (ELISA),) was used to determine the absorbance (OD) value at 450nm wavelength and to calculate the ALDH2 level of the sample. Results: 1the results of ALDH2 in acute myocardial infarction group, angina pectoris group and normal control group were 3.44 鹵1.53 鹵4.08 鹵1.85 鹵7.32 鹵0.34 U / L, respectively. The expression of ALDH2 was the lowest in AMI group, the highest in normal control group, followed by angina pectoris group. There was statistical difference between the AMI group and the normal control group (P value 0.05), and the angina pectoris group and the control group also had statistical significance (P value 0.05). However, there was no significant difference between AMI and angina pectoris. The results of 2.ALDH2 and coronary artery disease showed that the level of ALDH2 in three vessel lesions was higher than that in the other two groups, but there was a significant difference among the three groups. There was no significant difference between 3.ALDH2 and severity of coronary artery disease. The results showed that the ALDH2 level of the patients with severe lesions was the lowest, moderate, moderate. The results of 4.ALDH2 multiple linear regression analysis showed that hypertension and HDL of coronary heart disease had influence on the expression of ALDH2, and the difference was statistically significant. Univariate analysis showed that the levels of HDL and ALDH2 were correlated with the incidence of coronary heart disease in patients with coronary heart disease including AMI, angina pectoris group and experimental subjects (P 0.05), but had no statistical significance with other general indexes. Conclusion: the expression of 1.ALDH2 in angina pectoris group and acute myocardial infarction group is significantly lower than that in normal control group, suggesting that ALDH2 plays an important role in the pathogenesis of coronary heart disease. The level of 2.ALDH2 is negatively correlated with the severity of coronary artery disease. The level of 3.ALDH2 is related to the number of coronary artery disease branches. 4. The expression level of 3.ALDH2 in angina pectoris group and acute myocardial infarction group is significantly lower than that in normal control group. The results suggest that HDL and ALDH2 may play an important role in the pathogenesis of coronary heart disease.
【學位授予單位】：蚌埠醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R541.4
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