【摘要】：目的研究輕型地中海貧血成年男性精液質(zhì)量,探討精子γ-H2AX含量及其與精液參數(shù)、血清鐵蛋白濃度的關(guān)系。方法選取輕型地中海貧血的成年男性100例(輕型地中海貧血組)和確認有生育能力的健康男性100例(對照組)。通過檢測精子γ-H2AX含量、精子DNA損傷率、血清鐵蛋白濃度、精漿活性氧物質(zhì)(reactive oxygen species,ROS)及精液常規(guī)參數(shù),分析兩組間差異及輕型地中海貧血組精子γ-H2AX與精液參數(shù)、血清鐵蛋白濃度的相關(guān)性。結(jié)果相比對照組,輕型地中海貧血組的精子γ-H2AX表達率、精漿ROS、DNA損傷率及血清鐵蛋白濃度顯著增高(P0.001),而精子濃度、精子前向活動力及精子正常形態(tài)百分率顯著降低(P0.001);精子γ-H2AX表達率與精子DNA損傷率、精漿ROS水平、血清鐵蛋白濃度呈正相關(guān)(P0.001),而與精子前向活動力、精子正常形態(tài)百分率呈負相關(guān)(P0.001)。結(jié)論輕型地中海貧血成年男性的精液質(zhì)量較健康男性差,γ-H2AX含量明顯高于健康男性,這可能與鐵負荷增加所導致的精子DNA損傷率升高有關(guān)。
[Abstract]:Objective to study the semen quality of adult male patients with mild thalassemia and to explore the relationship between sperm 緯-H2AX content and semen parameters and serum ferritin concentration. Methods 100 adult males with mild thalassemia (mild thalassemia group) and 100 healthy men (control group) were selected. The sperm 緯-H2AX content, sperm DNA damage rate, serum ferritin concentration, seminal plasma reactive oxygen species (reactive oxygen species,ROS) and semen routine parameters were measured to analyze the difference between the two groups and the sperm 緯-H2AX and semen parameters in mild thalassemia group. Correlation of serum ferritin concentration. Results compared with the control group, the sperm 緯-H2AX expression rate, seminal plasma ROS,DNA damage rate and serum ferritin concentration in the mild thalassemia group were significantly higher than those in the control group (P0. 001). The percentage of forward motility and normal morphology of spermatozoa decreased significantly (P0.001). The expression rate of 緯-H2AX was positively correlated with sperm DNA damage rate, seminal plasma ROS level and serum ferritin concentration (P0. 001), but negatively correlated with sperm forward motility and the percentage of normal sperm morphology (P0. 001). Conclusion the semen quality of adult males with mild thalassemia is worse than that of healthy men, and the content of 緯-H2AX is significantly higher than that of healthy men, which may be related to the increase of DNA damage rate of sperm caused by the increase of iron load.
【作者單位】： 廣西醫(yī)科大學第一附屬醫(yī)院檢驗科;廣西醫(yī)科大學第一附屬醫(yī)院精子庫;廣西壯族自治區(qū)計劃生育中心男性科;廣西醫(yī)科大學地中海貧血實驗室;
【基金】：廣西科學研究與技術(shù)開發(fā)計劃項目課題(編號:桂科攻10124001A-45)
【分類號】：R446.19;R556.61

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