【摘要】：目的:觀察IGF-1對(duì)SD大鼠主動(dòng)脈弓動(dòng)脈粥樣硬化病變處的巨噬細(xì)胞、TNF-α和血管平滑肌細(xì)胞的影響,以探討其可能的抗動(dòng)脈粥樣硬化機(jī)制,為其臨床應(yīng)用提供理論依據(jù)和實(shí)踐指導(dǎo)。方法:共24只8周齡大小的SD大鼠,隨機(jī)分為模型組(12只)和IGF-1組(12只),分別測(cè)量它們的體重(單位為g)、血壓(單位為mmHg)及血脂(單位為mmol/L)水平作為基礎(chǔ)值。IGF-1組給予高脂飲食+腹腔注射IGF-1,所用劑量為1ml(0.1mg/ml),模型組給予高脂飲食+腹腔注射生理鹽水1.0ml,于高脂飼料喂養(yǎng)16周后再次測(cè)量剩余所有SD大鼠的體重、血壓及血脂水平,獲取最終剩余的兩組SD大鼠的主動(dòng)脈弓進(jìn)行病理學(xué)分析:HE染色觀察主動(dòng)脈弓橫斷面的動(dòng)脈粥樣硬化病變程度;免疫組化法半定量分別分析主動(dòng)脈弓橫斷面的動(dòng)脈粥樣硬化病變處TNF-α、巨噬細(xì)胞標(biāo)志物CD68及血管平滑肌細(xì)胞標(biāo)志物α-SMA各自的光密度值(Optical density,OD)。結(jié)果:與模型組相比,IGF-1組SD大鼠的體重、血壓及血脂無顯著差異(P0.05),巨噬細(xì)胞標(biāo)志物CD68及TNF-α各自的OD值水平顯著減少(P0.05),同時(shí)血管平滑肌細(xì)胞標(biāo)志物α-SMA的OD值顯著增加(P0.05)。結(jié)論:IGF-1可能通過減少動(dòng)脈粥樣硬化斑塊內(nèi)炎癥及炎癥因子水平、增加血管平滑肌細(xì)胞數(shù)量來發(fā)揮抗動(dòng)脈粥樣硬化作用。
[Abstract]:Objective: to observe the effect of IGF-1 on macrophage TNF- 偽 and vascular smooth muscle cells (VSMC) in atherosclerotic lesions of aortic arch of SD rats, and to explore its possible anti-atherosclerosis mechanism and to provide theoretical basis and practical guidance for its clinical application. Methods: a total of 24 8-week-old SD rats were used. They were randomly divided into model group (12 rats) and IGF-1 group (12 rats). Their body weight (g), blood pressure (mmHg) and blood lipid (mmol/L) level were measured as basic values respectively. IGF-1 group was given high fat diet intraperitoneal injection of IGF-1, the dose was used. For 1ml (0.1mg/ml), the model group was given a high-fat diet intraperitoneal injection of physiological saline 1.0 ml. After 16 weeks of high-fat diet feeding, the body weight of all the remaining SD rats was measured again. Blood pressure and blood lipid levels were obtained from the aortic arch of the remaining two groups of SD rats. The pathological analysis was performed to observe the degree of atherosclerosis on the cross section of the aortic arch. The optical density (OD) of TNF- 偽, macrophage marker CD68 and vascular smooth muscle cell marker 偽 -SMA in atherosclerotic lesions of the aortic arch were analyzed by immunohistochemistry. Results: compared with the model group, the weight, blood pressure and blood lipid of SD rats in IGF-1 group showed no significant difference (P0.05). The OD levels of macrophage markers CD68 and TNF- 偽 were significantly decreased (P0.05), while the OD value of vascular smooth muscle cell marker 偽 -SMA was significantly increased (P0.05). ConclusionTwo one IGF-1 may play an anti-atherosclerotic effect by reducing the levels of inflammation and inflammatory factors in atherosclerotic plaques and increasing the number of vascular smooth muscle cells.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R543.5

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