【摘要】：目的:使用高剪切乳化機制備全氟化碳乳劑(PFCE),通過靜脈注射PFCE對動脈粥樣硬化(AS)模型大鼠進行干預(yù),觀察其內(nèi)皮功能指標(biāo)的變化,初步探討PFCE對AS大鼠血管舒縮活性因子的調(diào)控作用及對內(nèi)皮的可能保護作用。方法:將50只Wistar雄鼠隨機分為5組:空白對照組,模型組,PFCE低、中、高劑量組,每組10只。除空白對照組用普通維持飼料外,其余均以維生素D3加高脂飼料喂養(yǎng)誘導(dǎo)實驗性大鼠AS模型,PFCE低、中、高劑量組在造模同時給予不同劑量藥物干預(yù)。12周后,測定各組大鼠血清NO、ET-1、TXA2、6-K-PG的含量,蘇木精-伊紅染色法觀察各組大鼠主動脈病理變化。結(jié)果:模型組NO、TXA2含量顯著降低,ET-1、6-K-PG含量顯著升高,與空白對照組比較差異有統(tǒng)計學(xué)意義(P0.05),且NO/ET-1比值、TXA2/6-K-PG比值平衡失調(diào);PFCE高、中劑量組較模型組血清NO、TXA2含量顯著升高,ET-1、6-K-PG含量顯著降低(P0.05),并改善NO/ET-1及TXA2/6-K-PG的比值(P0.05);PFCE低劑量組與模型組相比血清中各指標(biāo)差異無統(tǒng)計學(xué)意義(P0.05);PFCE高劑量組與PFCE低劑量組相比血清NO、TXA2含量顯著升高,ET-1、6-K-PG含量顯著降低(P0.05),與中等劑量組差異不顯著(P0.05);PFCE高、中劑量組較PFCE低劑量組減輕AS大鼠主動脈組織形態(tài)學(xué)變化更明顯。結(jié)論:中等劑量以上PFCE可能通過調(diào)控AS大鼠血管舒縮活性因子,起到保護血管內(nèi)皮的功能,發(fā)揮阻止AS進一步發(fā)生和發(fā)展的作用。
[Abstract]:Objective: to observe the changes of endothelial function in atherosclerotic (AS) model rats by intravenous injection of (PFCE), a perfluorocarbon emulsion prepared by high shear emulsifying machine. Objective: to investigate the regulatory effect of PFCE on vasomotor activity factor and its possible protective effect on endothelium in as rats. Methods: fifty male Wistar rats were randomly divided into 5 groups: blank control group, model group, low, medium and high dose groups with 10 rats in each group. With the exception of the blank control group, the rats were fed with vitamin D3 plus high fat diet to induce experimental as model with low PFCE. The middle and high dose groups were treated with different doses of drugs at the same time for 12 weeks. The contents of TXA2 6-K-PG and the pathological changes of aorta in each group were observed by hematoxylin and eosin staining. Results: the content of NOTXA2 in the model group was significantly lower than that in the control group, and the content of 6-K-PG was significantly higher than that of the control group (P0.05), and the ratio of NO/ET-1 to TXA _ 2 / 6-K-PG was not balanced significantly (P > 0.05), and the ratio of TXA _ 2 / 6-K-PG was higher than that of the control group. Compared with the model group, the serum NOTXA2 content in the middle dose group was significantly increased (P0.05), and the ratio of NO/ET-1 to TXA2/6-K-PG was improved (P0.05). There was no significant difference in serum indexes between the low dose group and the model group (P0.05). Compared with the middle dose group, the content of TXA2 in serum increased significantly (P0.05), the content of 6-K-PG decreased significantly (P0.05), but the level of PFCE was higher than that in the middle dose group (P0.05). The changes of aortic histomorphology in as rats were significantly reduced in middle dose group than in low dose PFCE group. Conclusion: the medium dose of PFCE may protect the endothelium and prevent the further development of as by regulating vasomotor activity factor in as rats.
【作者單位】： 青島大學(xué)醫(yī)學(xué)院心內(nèi)科;中國人民解放軍401醫(yī)院心內(nèi)科;
【分類號】：R543.5

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