【摘要】：通過構(gòu)建長期有氧運動模型,明確游泳運動對幼年自發(fā)性高血壓大鼠腓腸肌微血管稀少的作用并探討其機(jī)制。采用4周齡雄性大鼠,分為3組:對照組(WKY)、自發(fā)性高血壓組(SHR)及高血壓運動組(SE)。HE染色觀察腓腸肌微血管密度、微血管數(shù)目與肌細(xì)胞數(shù)目的比值及肌纖維橫截面積的變化,運用免疫組化方法和Western Blot方法檢測各組大鼠腓腸肌中VEGF、eNOS、PI3K、Akt的蛋白表達(dá)。實驗發(fā)現(xiàn):與WKY組相比,SHR組腓腸肌中微血管密度、微血管數(shù)目與骨骼肌細(xì)胞數(shù)目的比值、肌纖維橫截面積、腓腸肌總蛋白含量明顯減少(P0.01);8周游泳訓(xùn)練之后,SE組與SHR組相比,各項指標(biāo)明顯增加(P0.01)。免疫組化和Western Blot實驗結(jié)果顯示:與WKY組相比,SHR腓腸肌中的VEGF、PI3K、Akt、eNOS表達(dá)明顯下降,SE組腓腸肌中VEGF、PI3K、Akt、eNOS蛋白表達(dá)與SHR相比均有明顯增加;游泳運動增加了SHR腓腸肌中VEGF、PI3K、Akt、eNOS的蛋白表達(dá)。有氧運動可以改善幼年自發(fā)性高血壓大鼠腓腸肌中微細(xì)血管稀少的現(xiàn)象,其機(jī)制與運動增加VEGF及eNOS的表達(dá)及上調(diào)PI3K/Akt信號通路有關(guān)。
[Abstract]:The effects of swimming on vascularity of gastrocnemius muscle in young spontaneously hypertensive rats were studied by establishing a long-term aerobic exercise model. Four week-old male rats were divided into three groups: control group (WKY), spontaneous hypertension group (SHR) and hypertensive exercise group (SE). He staining was used to observe the changes of microvessel density, ratio of microvessel number to myocyte number and cross-sectional area of muscle fiber in gastrocnemius muscle. Immunohistochemical method and Western blot were used to detect the protein expression of VEGFERENOSN PI3KU Akt in gastrocnemius muscle of rats in each group. The results showed that compared with WKY group, the microvessel density in gastrocnemius muscle, the ratio of microvessel number to skeletal muscle cell number, the cross sectional area of muscle fiber and the total protein content of gastrocnemius muscle in SHR group were significantly decreased (P0.01). The indexes increased significantly (P0.01). The results of immunohistochemistry and Western Blot showed that the expression of VEGFFnPI3KP3KnAktOEnos in the gastrocnemius muscle of SHR was significantly lower than that in the WKY group, and the expression of VEGFFt3KPI3KPI3KPI3KPI3KnAktNs in the gastrocnemius muscle of the SE group was significantly higher than that in the SHR group, while swimming exercise increased the expression of VEGFFnPI3KU AktNs protein in the gastrocnemius muscle of the SHR compared with that of the SHR group. Aerobic exercise can improve the phenomenon of small blood vessel scarcity in gastrocnemius muscle of young spontaneously hypertensive rats, and its mechanism is related to the increase of VEGF and Enos expression and the up-regulation of PI3K / Akt signal pathway by exercise.
【作者單位】： 陜西師范大學(xué)體育學(xué)院;
【基金】：國家自然科學(xué)基金(31071035) 陜西省自然科學(xué)基金(2016JM3008)
【分類號】：R544.1

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