【摘要】：目的探討膠原纖維在風(fēng)濕性心臟瓣膜病心房顫動(dòng)(房顫)患者中的異常表達(dá)以及與房顫發(fā)生的相關(guān)性。方法 33例風(fēng)濕性心臟病接受換瓣患者分為竇性心律組與房顫組。于手術(shù)中獲取右心耳組織。采用轉(zhuǎn)錄組學(xué)進(jìn)行差異表達(dá)基因的功能分析;RT-PCR檢測(cè)Ⅰ~Ⅳ型膠原纖維RNA在右心房組織中的表達(dá);免疫組化觀察心肌組織各型膠原纖維的表達(dá)與分布。結(jié)果與竇性心律組相比,房顫組左心房?jī)?nèi)徑明顯擴(kuò)大。轉(zhuǎn)錄組學(xué)分析顯示COL1A2、COL2A1、COL3A1以及COL4A3與COL5A2、COL6A3、COL11A1等膠原纖維存在相互關(guān)聯(lián)之外,與多種細(xì)胞因子也存在相互作用;RT-PCR結(jié)果示COL1A2、COL2A1、COL3A1和COL4A3均在房顫患者右心房組織中上調(diào)表達(dá),其中COL3A1、COL4A3上調(diào)表達(dá)顯著(P0.05)。免疫組化結(jié)果顯示Ⅰ型膠原纖維分布在血管內(nèi)皮細(xì)胞及血管基底膜;Ⅱ型膠原纖維分布在心肌細(xì)胞肌節(jié);Ⅲ型膠原纖維分布在間皮細(xì)胞及心肌細(xì)胞漿;Ⅳ型膠原纖維分布在心肌細(xì)胞外膜。結(jié)論心房組織中COL3A1與COL4A3表達(dá)異常,與多種細(xì)胞因子相互作用引發(fā)房顫的發(fā)生和發(fā)展。
[Abstract]:Objective to investigate the abnormal expression of collagen fibers in patients with atrial fibrillation (AF) in rheumatic valvular heart disease. Methods 33 patients with rheumatic heart disease received valve replacement were divided into sinus rhythm group and atrial fibrillation group. Right atrial appendage tissue was obtained during operation. The functional analysis of differentially expressed genes was performed by transcriptome. RT-PCR was used to detect the expression of type 鈪，

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