本文選題：主動脈夾層 + 血管緊張素II��； 參考：《中國實驗動物學(xué)報》2017年04期

【摘要】：目的應(yīng)用β-氨基丙腈(β-aminopropionitrile,BAPN)飲水聯(lián)合血管緊張素II(angiotensin II,Ang II)皮下埋泵建立小鼠主動脈夾層模型。方法 40只3周齡雄性C57Bl/6J小鼠隨機分為兩組,均每日給予0.1 g/(kg·bw)BAPN飲水,BAPN飲水后第28天給予皮下埋泵,BAPN飲水+生理鹽水埋泵組持續(xù)泵入生理鹽水,BAPN飲水+Ang II埋泵組持續(xù)泵入Ang II,每分鐘1000 ng/(kg·bw)。采用小鼠無創(chuàng)血壓測量計尾套法檢測小鼠血壓和心率。實驗中死亡小鼠立即解剖,分離主動脈。埋泵72 h后,未死亡小鼠注射過量戊巴比妥鈉處死,留取主動脈。蘇木素-伊紅染色顯微鏡下觀察主動脈壁假腔形成情況。結(jié)果 BAPN飲水+Ang II埋泵組小鼠總的夾層發(fā)生率為95%,死亡率為24%(夾層動脈瘤破裂死亡),BAPN飲水+生理鹽水埋泵組夾層發(fā)生率為5%,死亡率為0;鏡下主動脈病理切片顯示夾層主動脈有假腔形成。結(jié)論成功建立高主動脈夾層發(fā)生率的小鼠模型。
[Abstract]:Objective to establish a murine aortic dissection model by using 尾 -aminopropionitrile bun (尾 -aminopropionitrile) drinking water and subcutaneous pump of angiotensin II (angiotensin II). Methods Forty 3-week-old male C57Bl6J mice were randomly divided into two groups. Every day, 0.1 g / (kg bw) BAPN drinking water and BAPN drinking water on the 28th day after drinking, the subcutaneous pump / BAPN drinking saline water buried pump group continued to pump into normal saline water Ang II drinking water pump group continued to pump into Ang II, 1000 ng/ (kg bw). Per minute. The blood pressure and heart rate of mice were measured by non-invasive blood pressure measurement. The dead mice were dissected immediately and the aorta was separated. After 72 hours, the undead mice were killed by injection of excessive pentobarbital sodium, and the aorta was retained. Hematoxylin-eosin staining microscope was used to observe the formation of false lumen in aortic wall. Results the incidence of dissection was 95% and the mortality rate was 24% (rupture of dissecting aneurysm) in the Ang II drinking water pump group. The incidence of dissection was 5 and the mortality was 0 in BAPN drinking water saline embedding pump group, and the pathological section of aorta was observed under microscope. Dissecting aorta showed false lumen formation. Conclusion the mouse model with high incidence of aortic dissection was successfully established.
【作者單位】： 北京大學(xué)中日友好臨床醫(yī)學(xué)院;中日友好醫(yī)院心內(nèi)科;中國醫(yī)學(xué)科學(xué)院北京協(xié)和醫(yī)學(xué)院中日友好臨床醫(yī)學(xué)院;中日友好醫(yī)院心血管病中心研究室;中日友好醫(yī)院臨床醫(yī)學(xué)研究所生物醫(yī)學(xué)實驗研究平臺;
【基金】：國家自然科學(xué)基金項目(編號:81170287,81500326)
【分類號】：R-332;R543.1
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本文編號：2116267