本文選題：miR- + 下肢動(dòng)脈硬化閉塞癥��； 參考：《中國(guó)動(dòng)脈硬化雜志》2017年08期

【摘要】：目的觀察microRNA-4463(miR-4463)在下肢動(dòng)脈硬化閉塞癥(ASO)患者血漿和組織中的表達(dá)變化,推測(cè)其可能意義。方法采用miRNA芯片方法篩選ASO患者和對(duì)照人群血漿中差異表達(dá)miRNA,Real time PCR技術(shù)驗(yàn)證ASO患者和對(duì)照人群中miR-4463的表達(dá)水平,并進(jìn)行臨床分期比較。靶基因預(yù)測(cè)軟件分析miR-4463靶基因,Gene Ontology和KEGG數(shù)據(jù)庫(kù)分析靶基因的功能和信號(hào)通路。結(jié)果與對(duì)照組相比,ASO患者血漿中有51個(gè)miRNA變化超過(guò)1.5倍(P0.05)。miR-4463在ASO患者血漿和病變血管內(nèi)膜組織中表達(dá)均顯著降低,并隨Fontaine分期呈逐漸下降趨勢(shì)。生物信息學(xué)分析發(fā)現(xiàn)miR-4463靶基因與細(xì)胞遷移、脂質(zhì)代謝、內(nèi)吞作用等相關(guān)。結(jié)論 miR-4463參與了ASO的病程,其表達(dá)下降可能提示ASO的發(fā)生。
[Abstract]:Objective To observe the changes in the expression of microRNA-4463 (miR-4463) in the plasma and tissue of patients with arteriosclerotic occlusive disease (ASO) of the lower extremities, and to speculate on its possible significance. Methods the differential expression of miRNA in the plasma of ASO patients and the control population was screened by miRNA chip method. The Real time PCR technique was used to verify the expression level of miR-4463 in the ASO patients and the control population. The target gene prediction software analyzed the miR-4463 target gene, the Gene Ontology and the KEGG database to analyze the function and signal pathway of the target gene. Results compared with the control group, 51 miRNA changes in the plasma of ASO patients were more than 1.5 times (P0.05).MiR-4463 in the plasma of ASO patients and the intima tissue of the lesion vessels significantly. Fontaine staging was gradually decreasing. Bioinformatics analysis found that the miR-4463 target gene was related to cell migration, lipid metabolism, endocytosis, etc. conclusion miR-4463 participates in the course of ASO, and the decline of its expression may suggest the occurrence of ASO.
【作者單位】： 西南醫(yī)科大學(xué)附屬醫(yī)院醫(yī)學(xué)實(shí)驗(yàn)中心;西南醫(yī)科大學(xué)臨床醫(yī)學(xué)系;西南醫(yī)科大學(xué)附屬醫(yī)院血管外科;
【基金】：四川省教育廳科研項(xiàng)目(16ZA0179) 瀘州市-瀘州醫(yī)學(xué)院聯(lián)合課題(LZ-LY-20)
【分類號(hào)】：R543.5
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本文編號(hào)：2082047