本文選題：腎血管性高血壓大鼠 + 基質(zhì)金屬蛋白酶　； 參考：《中國實(shí)驗(yàn)動(dòng)物學(xué)報(bào)》2017年02期

【摘要】：目的探討腎血管性高血壓大鼠(RHR)血清、腦及血管組織中基質(zhì)金屬蛋白酶9(MMP-9)和組織型基質(zhì)金屬蛋白酶抑制因子1(TIMP-1)的表達(dá)以及血壓與兩者的關(guān)系。方法雄性SD大鼠80只隨機(jī)分成RHR組(40只)和假手術(shù)組(40只)。RHR組采用雙腎-雙夾法制作高血壓大鼠模型,鼠尾測(cè)壓儀測(cè)定血壓。根據(jù)Longa5評(píng)分法和病理學(xué)結(jié)果確定腦卒中;Western blot和免疫組化測(cè)定腦組織和血管中MMP-9和TIMP-1的表達(dá),雙抗體夾心ELISA檢測(cè)血清MMP-9和TIMP-1水平。結(jié)果與假手術(shù)組比較,RHR組大鼠術(shù)后2、4、6、8、10、12周血壓均明顯升高[(157±9.0)比(128±7.0),(176±10.0)比(122±6.0),(194±8.0)比(117±6.5),(202±12.0)比(124±8.0),(218±15.0)比(126.±8.5),(224±20.0)比(129.±9.0)mm Hg,均P0.05];術(shù)后12周,RHR大鼠血清MMP-9高于假手術(shù)組[(783.4±109.79)比(573.4±109.59)ng/m L,P0.05];而RHR大鼠血清TIMP-1低于假手術(shù)組[(313.02±83.9)比(976.19±191.1)pg/m L,P0.05]。同時(shí),RHR大鼠腦組織和血管MMP-9的表達(dá)均明顯高于假手術(shù)組(均P0.05),而TIMP-1的表達(dá)則均明顯低于假手術(shù)組(均P0.05)。Pearson相關(guān)分析顯示收縮壓與血清、腦及血管組織中的MMP-9水平均呈正相關(guān)(r=0.557,r=0.774和r=0.661,均P0.05),而與TIMP-1水平均呈負(fù)相關(guān)(r=-0.481,r=-0.535和r=-0.685,均P0.01)。結(jié)論腎血管性高血壓大鼠血清、腦和血管組織中MMP-9表達(dá)升高,而TIMP-1表達(dá)降低。兩者在血液和組織中的變化趨勢(shì)保持一致,血壓的升高與血液和組織中MMP-9的升高和TIMP-1的降低相關(guān)。
[Abstract]:Objective to investigate the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in serum, brain and vascular tissues of renovascular hypertensive rats (RHR) and the relationship between blood pressure and the expression of MMP-9 and TIMP-1. Methods 80 male Sprague-Dawley rats were randomly divided into two groups: RHR group (n = 40) and sham operation group (n = 40). The expressions of MMP-9 and TIMP-1 in brain tissue and blood vessel were determined by immunohistochemistry and Western blot according to the results of Longa5 score and pathology. The levels of MMP-9 and TIMP-1 in serum were detected by double antibody sandwich Elisa. 緇撴灉涓庡亣鎵嬫湳緇勬瘮杈，

本文編號(hào)：2062012