本文選題：骨內(nèi)注射 + 辛伐他汀��； 參考：《中國實驗動物學(xué)報》2017年05期

【摘要】：目的研究骨內(nèi)局部單次注射小劑量辛伐他汀對大鼠心梗后血管新生和心功能的影響。方法Wistar大鼠隨機分為假手術(shù)組、心肌梗死模型組和骨內(nèi)注射辛伐他汀組(n=12)。冠狀動脈左前降支結(jié)扎建立大鼠心肌梗死模型。24 h后實驗組左脛骨內(nèi)單次注射辛伐他汀0.5 mg,4周后分別通過小動物超聲心動圖評價左室功能,三苯基氯化四氮唑(TTC)染色計算心肌梗死面積,免疫熒光染色檢測局部血管新生情況。結(jié)果超聲心動圖結(jié)果表明心肌梗死4周后左心室收縮功能明顯下降,骨內(nèi)注射辛伐他汀對大鼠心肌梗死后左心室功能未見明顯改善;TTC染色發(fā)現(xiàn)骨內(nèi)注射辛伐他汀組心肌梗死面積未見明顯減少;免疫熒光染色顯示,骨內(nèi)注射辛伐他汀組心肌血管密度沒有顯著增加。結(jié)論大鼠心梗24 h后骨內(nèi)單次注射小劑量辛伐他汀(0.5 mg),心肌梗死面積、血管新生及心臟功能無顯著改善。
[Abstract]:Objective to study the effect of single intraosseous injection of simvastatin on angiogenesis and cardiac function after myocardial infarction in rats. Methods Wistar rats were randomly divided into sham-operation group, myocardial infarction model group and intraosseous simvastatin group. Rat myocardial infarction model was established by ligation of left anterior descending coronary artery. The left ventricular function was evaluated by small animal echocardiography 4 weeks after single injection of simvastatin 0.5 mg / g into left tibia. The size of myocardial infarction was calculated by TTC staining and local angiogenesis was detected by immunofluorescence staining. Results the results of echocardiography showed that left ventricular systolic function decreased significantly 4 weeks after myocardial infarction. Intraosseous injection of simvastatin did not significantly improve left ventricular function after myocardial infarction in rats. TTC staining showed that the area of myocardial infarction was not significantly decreased in the group of intraosseous injection of simvastatin, and immunofluorescence staining showed that, There was no significant increase in myocardial vascular density after intraosseous injection of simvastatin. Conclusion single intraosseous injection of simvastatin (0.5 mg / g) at 24 hours after myocardial infarction has no significant improvement on myocardial infarction size, angiogenesis and cardiac function.
【作者單位】： 北京大學(xué)第三醫(yī)院骨科;脊柱疾病研究北京市重點實驗室;北京大學(xué)第三醫(yī)院心內(nèi)科;北京大學(xué)第三醫(yī)院神經(jīng)內(nèi)科;
【基金】：國家自然科學(xué)基金(NO.81672133,81641079) 國家高技術(shù)研究發(fā)展計劃(863計劃NO.2015AA020304)
【分類號】：R542.22

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